Large-scale apple GWAS reveals NAC18.1 as a master regulator of ripening traits

Q4 Agricultural and Biological Sciences

Fruit Growing Research Pub Date : 2023-01-01 DOI:10.48130/frures-2023-0032

Sophie Watts, Zoë Migicovsky, Sean Myles

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引用次数: 0

Abstract

Apple quality traits such as fruit texture, sugar content, and firmness retention during storage are key targets for breeders. Understanding the genetic control of fruit quality traits can enable the development of genetic markers, useful for marker-assisted breeding of new apple cultivars. We made use of over 260,000 single nucleotide polymorphisms (SNPs) genotyped across 1,054 apple accessions from Canada's Apple Biodiversity Collection to perform genome-wide association for 21 fruit quality and phenology traits. We identified two loci on chromosome 15 and 16 associated with phenolic content and a locus on chromosome 10 associated with softening. In addition, we determined that allelic variation at the NAC18.1 transcription factor was associated with numerous traits including harvest date, firmness at harvest, and firmness after storage. Our analyses suggest that NAC18.1 independently acts as a high level regulator of multiple ripening related traits and we propose a model for the allelic effects at NAC18.1 on apple ripening and softening.

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大规模苹果GWAS显示NAC18.1作为成熟性状的主要调节者

苹果品质性状，如果实质地、含糖量和贮藏期间的硬度是育种者的关键目标。了解果实品质性状的遗传控制，可以开发遗传标记，为苹果新品种的标记辅助育种提供依据。利用来自加拿大苹果生物多样性数据库的1054份苹果材料中超过26万个单核苷酸多态性(SNPs)进行基因分型，对21个果实品质和物候性状进行全基因组关联。我们在第15和16号染色体上发现了两个与酚含量有关的位点，在第10号染色体上发现了一个与软化有关的位点。此外，我们确定NAC18.1转录因子的等位基因变异与许多性状有关，包括收获日期、收获时的硬度和储存后的硬度。我们的分析表明，NAC18.1在苹果成熟和软化过程中具有高水平的调控作用，并建立了NAC18.1的等位基因效应模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fruit Growing Research Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

0.10

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： FRUIT GROWING RESEARCH is an international journal, which has been issued under the care of the Research Institute for Fruit Growing Pitesti, Romania. Fruit growing research is an annual journal aimed at disseminating significant research and original papers on genetic resources, breeding of deciduous fruit trees species; fruit sustainable technologies – environment protection; propagation, virology and tissue culture, postharvest, that can be of interest to a wide audience of plant scientists in all areas of fruit growing. An international Editorial Board advises the journal.

Large-scale apple GWAS reveals <i>NAC18.1</i> as a master regulator of ripening traits

Abstract