{"title":"Histopathological and Immunohistochemical Study of Peri-Implant Epithelium","authors":"Yoshikazu Nakayama, Chieko Taguchi, Mitsuko Nakayama, Masaaki Suemitsu, Kayo Kuyama","doi":"10.4236/ojst.2023.139025","DOIUrl":null,"url":null,"abstract":"Background: Human beta-defensins (HBDs), epithelial-derived antimicrobial peptides, contribute to gingival health and periodontal disease. Particularly, the upregulation of HBD-3 expression in periodontal tissues is described to contribute to periodontal tissue regeneration. Specifically, the upregulation of HBD-3 expression in periodontal tissues is described to contribute to periodontal tissue regeneration. But there has been insufficient investigation of the inner epithelium in peri-implantitis, especially the HBD expression patterns as protection against oral infection. Objectives: The purpose of this study was to investigate the histopathological characteristics of the inner implant epithelium in peri-implantitis using histopathological and immunohistochemical methods and to compare it with the inner marginal epithelium in periodontitis. Materials and Methods: The biopsied cases consisted of 10 peri-implantitis, 11 periodontitis, and 10 controls. HE observation, measurement of the thickness of the inner epithelium, and immunohistochemical analysis for Ki-67 and HBD-3 were conducted. Results: Concerning the thickness of the inner epithelium, it was significantly higher in periodontitis (156.2 [138.0, 186.4] μm) than in peri-implantitis and control, 70.7 [67.5, 97.5] μm and 80.7 [76.6, 89.4] μm, respectively (p < 0.001). The Ki-67 positivity rates, both for inner epithelium and outer epithelium, were significantly higher in the following order: periodontitis, peri-implantitis, and control (p < 0.001). The inner epithelium is significantly elevated in the case of periodontitis (p < 0.001). For HBD-3, the intensity and region score (IRS) was significantly higher for inner epithelium than for outer epithelium (superficial layer) (p < 0.001) in all cases. The comparisons of IRS, significantly higher values were seen in inner epithelium of periodontitis than that of peri-implantitis (p < 0.001). Conclusion: Decreased production of HBD-3 in keratinocytes composing the inner implant epithelium may be one of the factors affecting tissue repair in peri-implantitis.","PeriodicalId":56569,"journal":{"name":"口腔学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"口腔学期刊(英文)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4236/ojst.2023.139025","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Human beta-defensins (HBDs), epithelial-derived antimicrobial peptides, contribute to gingival health and periodontal disease. Particularly, the upregulation of HBD-3 expression in periodontal tissues is described to contribute to periodontal tissue regeneration. Specifically, the upregulation of HBD-3 expression in periodontal tissues is described to contribute to periodontal tissue regeneration. But there has been insufficient investigation of the inner epithelium in peri-implantitis, especially the HBD expression patterns as protection against oral infection. Objectives: The purpose of this study was to investigate the histopathological characteristics of the inner implant epithelium in peri-implantitis using histopathological and immunohistochemical methods and to compare it with the inner marginal epithelium in periodontitis. Materials and Methods: The biopsied cases consisted of 10 peri-implantitis, 11 periodontitis, and 10 controls. HE observation, measurement of the thickness of the inner epithelium, and immunohistochemical analysis for Ki-67 and HBD-3 were conducted. Results: Concerning the thickness of the inner epithelium, it was significantly higher in periodontitis (156.2 [138.0, 186.4] μm) than in peri-implantitis and control, 70.7 [67.5, 97.5] μm and 80.7 [76.6, 89.4] μm, respectively (p < 0.001). The Ki-67 positivity rates, both for inner epithelium and outer epithelium, were significantly higher in the following order: periodontitis, peri-implantitis, and control (p < 0.001). The inner epithelium is significantly elevated in the case of periodontitis (p < 0.001). For HBD-3, the intensity and region score (IRS) was significantly higher for inner epithelium than for outer epithelium (superficial layer) (p < 0.001) in all cases. The comparisons of IRS, significantly higher values were seen in inner epithelium of periodontitis than that of peri-implantitis (p < 0.001). Conclusion: Decreased production of HBD-3 in keratinocytes composing the inner implant epithelium may be one of the factors affecting tissue repair in peri-implantitis.