Microbial production of zeaxanthin from algal biomass and waste glycerol using a newly isolated agarolytic marine bacterium Cellulophaga omnivescoria MSK1

IF 4 4区环境科学与生态学 Q2 ENVIRONMENTAL STUDIES

Energy & Environment Pub Date : 2023-10-03 DOI:10.1177/0958305x231204032

Min Sun Kim, Sun-Wook Jeong, Jung Eun Yang, Yong Jun Choi

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引用次数: 0

Abstract

With increasing interests in the biological conversion of waste resources into value-added chemicals and biocompounds, algal biomass has emerged as a promising renewable feedstock due to abundance, rapid growth, and sustainability. However, efficient bioconversion method is still being challenged due to the lack of suitable host strain capable of directly utilizing algal biomass and converting into desired products. In this study, a potent marine bacterium capable of producing zeaxanthin from algal biomass was investigated. The agarolytic bacterium, Cellulophaga omnivescoria MSK1 was isolated from coastal seawater. Through physiological and morphological analysis, it was confirmed that this bacterium degrade agarose into neoagarotetraose (NA4) and neoagarohexaose (NA6) and produces zeaxanthin using agarose as a carbon source. This bacterial strain demonstrated the ability to produce 2.23 ± 0.19 mg/L of zeaxanthin when provided with 0.2% agarose as a carbon source. By supplementing with glycerol, zeaxanthin production was further enhanced, reaching levels of 8.43 ± 0.31 mg/L, with a content of 6.77 ± 0.3 mg/g CDW (cell dry weight). These results suggest that an agarolytic Cellulophaga omnivescoria MSK1 strain has a great potential to be used as a platform microbial strain capable of producing zeaxanthin from algal biomass as a sustainable feedstock.

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利用一种新分离的海洋溶脂细菌omnivescoria MSK1从藻类生物量和废甘油中生产玉米黄质

随着人们对将废弃资源转化为增值化学品和生物化合物的兴趣日益浓厚，藻类生物量因其丰富、快速生长和可持续性而成为一种有前途的可再生原料。然而，由于缺乏能够直接利用藻类生物量并转化为所需产品的合适宿主菌株，有效的生物转化方法仍然受到挑战。在这项研究中，研究了一种能够从藻类生物量中生产玉米黄质的强效海洋细菌。摘要从沿海海水中分离到一株广角纤维素菌MSK1。通过生理和形态分析，证实该细菌可将琼脂糖降解为新琼脂四糖(NA4)和新琼脂己糖(NA6)，并以琼脂糖为碳源生产玉米黄质。以0.2%琼脂糖为碳源时，该菌株的玉米黄质产率为2.23±0.19 mg/L。添加甘油可进一步提高玉米黄质产量，达到8.43±0.31 mg/L，细胞干重为6.77±0.3 mg/g CDW。综上所述，一株全孢噬纤维素菌(Cellulophaga omnivescoria) MSK1具有很大的潜力，可以作为一种平台微生物菌株，利用藻类生物质作为可持续原料生产玉米黄质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Energy & Environment ENVIRONMENTAL STUDIES-

CiteScore

7.60

自引率

7.10%

发文量

157

期刊介绍： Energy & Environment is an interdisciplinary journal inviting energy policy analysts, natural scientists and engineers, as well as lawyers and economists to contribute to mutual understanding and learning, believing that better communication between experts will enhance the quality of policy, advance social well-being and help to reduce conflict. The journal encourages dialogue between the social sciences as energy demand and supply are observed and analysed with reference to politics of policy-making and implementation. The rapidly evolving social and environmental impacts of energy supply, transport, production and use at all levels require contribution from many disciplines if policy is to be effective. In particular E & E invite contributions from the study of policy delivery, ultimately more important than policy formation. The geopolitics of energy are also important, as are the impacts of environmental regulations and advancing technologies on national and local politics, and even global energy politics. Energy & Environment is a forum for constructive, professional information sharing, as well as debate across disciplines and professions, including the financial sector. Mathematical articles are outside the scope of Energy & Environment. The broader policy implications of submitted research should be addressed and environmental implications, not just emission quantities, be discussed with reference to scientific assumptions. This applies especially to technical papers based on arguments suggested by other disciplines, funding bodies or directly by policy-makers.