Proteomic analysis of higher & lower altitude cultivars of Coffea arabica reveals differences related to environmental adaptations and coffee bean flavour.
Caitlin Fenrich, Phil Lauman, Prabashi Wickramasinghe
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引用次数: 0
Abstract
Coffee ranks among the most popular beverages worldwide and is an important commodity in developing nations. While coffee beans harvested from Coffea arabica are considered to have a superior rich and balanced flavour, they are susceptible to disease and climatic variables like temperature, precipitation, and oxygen availability, each of which varies with altitude. We performed a comprehensive proteomic comparison of two C. arabica cultivars, the high-altitude Rwanda Shyira (RS) and the lower-altitude Brazil Flor de Ipe (BFDI), using liquid chromatography MS/MS analysis. Five of the identified 531 proteins exhibited statistically significant differences in expressional intensity between the two cultivars. These differences may correspond to bitter flavonoid concentrations along with adaptations to cold, hypoxic, and disease stressors at different altitudes and geographic niches. These substantial proteomic differences identified between these elevations provide a greater understanding of the effects of altitude on the C. arabica plant and its coffee, which has implications for the global market.
咖啡是世界上最受欢迎的饮料之一,也是发展中国家的重要商品。虽然从阿拉比卡咖啡豆中收获的咖啡豆被认为具有优越的丰富和平衡的味道,但它们容易受到疾病和气候变量的影响,比如温度、降水和氧气的可用性,这些都随着海拔的变化而变化。采用液相色谱-质谱/质谱分析方法,对高海拔的卢旺达Shyira (RS)和低海拔的巴西Flor de Ipe (BFDI)两种阿拉比卡咖啡品种进行了蛋白质组学比较。所鉴定的531个蛋白中,有5个蛋白在两个品种之间的表达强度存在统计学差异。这些差异可能对应于苦味类黄酮浓度以及对不同海拔和地理位置的寒冷、缺氧和疾病压力源的适应。这些海拔高度之间确定的蛋白质组学差异提供了对海拔对阿拉比卡咖啡植物及其咖啡的影响的更好理解,这对全球市场具有重要意义。