The effect of zinc complex of N-isopropenylimidazole on the morphological characteristics of gum tissues in experimental endodontic-periodontal lesions in rats

Q3 Pharmacology, Toxicology and Pharmaceutics
Pavel A. Galenko-Yaroshevsky, Aleksandr А. Slavinskiy, Sergey S. Todorov, Viktor L. Popkov, Olga V. Shelemekh, Svetlana A. Lebedeva, Andrey V. Zadorozhniy, Anait V. Zelenskaya, Lusine O. Alukhanyan, Irina B. Nektarevskaya, Natalia D. Bunyatyan, Aleksandr А. Verevkin
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引用次数: 0

Abstract

Introduction: Combined inflammatory and destructive processes affecting the dental pulp and tissues of the periodontal complex are among the most problem diseases of the dental system. Current therapy with use of available pharmacological agents does not always allow achieving the expected positive result. In addition, often the lack of information about morphological processes in the tissues of the dental system, in particular the gums, with endodontic-periodontal lesion (EPL) limits the ability of dentists to carry out targeted pharmacotherapy with both traditional and, in particular, new medications. The aim of the study was to evaluate the morphological characteristics of gum tissues in a therapeutic context of N-isopropenylimidazole zinc complex derivative in experimental endodontic-periodontal lesion in rats. Materials and Methods: A simulation of EPL in rats was performed in two ways: simultaneous induction of acute periodontitis and parodontitis by pulp extraction and natural infection of the pulp cavity, as well as by ligation of the necks of lower incisors. The research protocols included 5 groups of animals: 1st – intact group (control-1); 2nd – animals with simulated EPL (control-2); 3rd – animals with simulated EPL and treated with Metrogyl Denta® gel (M-D); 4th – animals with simulated EPL and treated with N–isopropenylimidazole zinc complex derivative gel under the laboratory code Pilim-1; and 5th – animals with simulated EPL and treated with the combination of M-D + Pilim-1. The gum of the lower incisors was taken for morphological studies. Slides were stained with hematoxylin and eosin. Computer morphometry was performed using the ImageJ software. Results and Discussion: The substances M-D, Pilim-1 and, especially, the combination of M-D+Pilim-1 (against the background of chlorhexidine bigluconate used as oral rinse) for 14 days in rats with simulated EPL cause a significant improvement of the morphological structure of the gum with minimal residual dystrophy and sclerosis. The combination M-D + Pilim-1 led to a 1.3-time increase in epithelial thickness, and a 1.5-time decrease in acanthosis depth in comparison with M-D, while the number of capillaries and their diameter had no significant differences. Compared with Pilim-1, the epithelial thickness increased 1.5 times, and the acanthosis depth and the number of capillaries decreased 1.6 and 1.4 times, respectively, whereas the diameter of the capillaries did not change significantly. The pronounced protective effect of the combination M-D + Pilim-1 on the morphological structure of the gingival mucosa of rats with simulated EPL may be associated with antimicrobial, anti-inflammatory, regenerating, angioprotective and antioxidant properties of both M-D and Pilim-1 separately, and, possibly to a greater extent, of the combination M-D + Pilim-1. Conclusion: The substances M-D, Pilim-1 and, especially, the combination M-D + Pilim-1 (against the background of chlorhexidine bigluconate used as oral rinse) for 14 days in rats with simulated EPL have a protective effect on the epithelial structure and the connective tissue of the proper mucous plate, manifested in active normalization of pathological changes and significant restoration of their organotypic structure.
n -异丙烯咪唑锌配合物对实验性牙髓-牙周病变大鼠牙龈组织形态特征的影响
简介:影响牙髓和牙周复合体组织的炎症和破坏过程是牙齿系统中最棘手的疾病之一。目前使用现有药物的治疗并不总能达到预期的阳性结果。此外,牙髓-牙周病变(EPL)通常缺乏牙齿系统组织(特别是牙龈)形态学过程的信息,这限制了牙医使用传统药物和特别是新药物进行靶向药物治疗的能力。本研究的目的是评价n -异丙烯咪唑锌复合物衍生物治疗实验性牙髓-牙周病变大鼠牙龈组织的形态学特征。材料与方法:采用拔牙法和牙髓腔自然感染法、结扎下门牙颈法同时诱导急性牙周炎和牙周炎两种方法模拟大鼠EPL。研究方案分为5组动物:第一组为完整组(control-1);第二组是模拟EPL的动物(对照组-2);第3组:模拟EPL,经M-D凝胶治疗的动物;4 -模拟EPL动物,用n -异丙烯咪唑锌络合衍生物凝胶处理,实验室代码为Pilim-1;第5组为模拟EPL, M-D + Pilim-1联合用药。取下门牙牙龈进行形态学研究。切片用苏木精和伊红染色。使用ImageJ软件进行计算机形态测量。结果与讨论:M-D、匹利姆-1,特别是M-D+匹利姆-1联合(以双酚酸氯己定作为口腔冲洗液为背景)治疗模拟EPL大鼠14天,牙龈形态结构明显改善,营养不良和硬化残留极少。M-D + Pilim-1联合用药导致上皮厚度比M-D增加1.3倍,棘层深度比M-D减少1.5倍,而毛细血管数量及其直径无显著差异。与Pilim-1相比,上皮厚度增加了1.5倍,棘层深度和毛细血管数量分别减少了1.6倍和1.4倍,而毛细血管直径变化不显著。M-D + Pilim-1联合应用对模拟EPL大鼠牙龈黏膜形态结构的显著保护作用可能与M-D和Pilim-1的抗菌、抗炎、再生、血管保护和抗氧化特性有关,并且可能在更大程度上与M-D + Pilim-1联合应用有关。结论:M-D、匹利姆-1,特别是M-D +匹利姆-1联合(以双酚酸氯己定为口腔冲洗液为背景)对模拟EPL大鼠的上皮结构和适当粘膜板结缔组织具有保护作用,表现为病理改变积极正常化,器官型结构明显恢复。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Research Results in Pharmacology
Research Results in Pharmacology Medicine-Pharmacology (medical)
CiteScore
1.50
自引率
0.00%
发文量
32
审稿时长
12 weeks
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