Effect of impregnated valorised biogenic apatite with microbial culture filtrates on seed germination and seedling growth of mustard (Brassica juncea L.)

Abstract

Oliseed crops (Brassica napus and B. juncea) are the most important rabi oilseeds in India, following groundnut in the oilseed economy. The present study aimed to examine differential response of culture filtrates of phosphate solubilising microbes enriched with bonemeal to improve seed germination and seedling vigour of mustard. The culture filtrates of each organism was prepared by using PVK broth medium and using bonemeal as a P-source which again supplemented with chlorides and sulphates, respectively. The seeds of mustard were treated in each culture filtrate and kept overnight and then next day arranged in germination paper containing 200 seeds each. Seeds were soaked in sterilized water only serve as control. In experimental results maximum P-solubilization efficiency was shown by A. niger followed by B. subtillis, B. amyloliquifaciens, V. laecani and T. harzianum in broth. As the P-solubilization efficiency of microbes also increases gradually increases doses of bone meal. It is also found that the seed treated with culture filtrate obtained from Cl- and SO42- media containing 5g and 15g of bone meal which were inoculated with organisms shown very reliable progress which was recorded on germination percentage, seedling vigour, seedling length and root length.