Anti-Cancer Effects of Methanolic Extract of Toad Egg on Experimental Model

Abstract

Cancer is a complex multi-factorial disease of the cell. Drug discovery against cancer is ventured throughout the world especially from the nature products. Various drugs have been used in the treatment of cancer, but all of them have advantages and also different disadvantages. So, nowadays it is trying to identify different compounds from natural products which have anticancer properties. The objective(s) of this present study was to identify the anti-cancer activity of Bufo melanostictus Schneider, 1799, egg methanolic extract [TEE(M)] on the Ehrlich ascites carcinoma (EAC) cells in mice and also on human leukemic cell line (U937 and K562). Various experimental studies such as mean survival time study, U937 and K562 Cell proliferation study, bright field microscopy study, fluorescent microscopy study and MTT assay of U937 andK562 cells were performed. The results of these studies established that TEE(M) not only decreases EAC cell count but also reduces the EAC cell size and causes the apoptosis of the EAC cells. TEE(M) also decreases human myeloid leukemic cell count (U937 and K562) significantly. So, it can be said that this exact has significant anticancer effects.