Species delimitation and molecular phylogeny of the grasshopper subfamily Gomphocerinae (Orthoptera: Acrididae) from Algeria based on mitochondrial and nuclear DNA markers

IF 0.6 4区生物学 Q4 ZOOLOGY

African Zoology Pub Date : 2023-11-06 DOI:10.1080/15627020.2023.2263498

Rachida Hafayed, Abdelhamid Moussi, Huihui Chang, Yuan Huang

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引用次数: 0

Abstract

AbstractGomphocerinae grasshoppers (Orthoptera: Acrididae) are generally characterised by their polymorphism and cryptic diversity, which can be confusing when relying on morphological identification alone. DNA taxonomy serves as a powerful molecular tool for species identification and biodiversity assessment. In the context of zoogeography and the biodiversity conservation of animal resources in ecosystems, DNA barcoding data for Algerian Gomphocerinae fauna remains limited in global databases, despite their agroeconomic and environmental importance. Therefore, in this study, we collected various species of this subfamily from the Biskra region in Algeria and conducted DNA barcoding analysis, employing different molecular species delimitation methods (the automatic barcode gap discovery, assemble species by automatic partitioning, single-threshold general mixed Yule coalescent model, Bayesian Poisson tree process and multi-rate Poisson tree process methods), as well as phylogenetic analyses (maximum-likelihood and Bayesian inference) based on two molecular markers (COI and ITS). The results of the 163 newly generated sequences demonstrated that DNA barcoding technology is highly efficient and valuable for species identification in the subfamily Gomphocerinae, showing strong congruence with morphological evidence for 12 species, including eight species that were sequenced for the first time. This study also reported a new record of the genus Stenohippus Uvarov, 1926 in Algeria, comprising two species. Additionally, taxonomic revision allowed the species Dociostaurus biskrensis Moussi & Petit, 2014, to be transferred to the genus Stenohippus. Molecular tree analyses revealed the phylogenetic positions of the newly sequenced species within the subfamily Gomphocerinae and provided insights into their evolutionary relationships. These new data serve as a starting point for future research in other geographical areas, enabling a better understanding of the biodiversity of this insect group in Algeria.Keywords: BiskraCOI and ITSDNA barcodingmolecular taxonomynew recordStenohippus

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阿尔及利亚蝗虫亚科(直翅目:蝗科)的线粒体和核DNA标记的种界和分子系统发育

摘要:蝗科(直翅目:蝗科)具有多态性和隐型多样性的特点，单靠形态鉴定容易造成混淆。DNA分类学是物种鉴定和生物多样性评价的有力分子工具。在动物地理学和生态系统动物资源生物多样性保护的背景下，尽管阿尔及利亚Gomphocerinae动物群具有重要的农业经济和环境意义，但其DNA条形码数据在全球数据库中仍然有限。因此，在本研究中，我们从阿尔及利亚Biskra地区收集了该亚科的多个物种，并采用不同的分子物种划分方法(自动条形码间隙发现、自动划分组装物种、单阈值一般混合Yule聚结模型、贝叶斯泊松树过程和多速率泊松树过程方法)进行DNA条形码分析。以及基于两个分子标记(COI和ITS)的系统发育分析(最大似然和贝叶斯推理)。163个新生成的序列结果表明，DNA条形码技术在Gomphocerinae亚科的物种鉴定中具有很高的效率和价值，与形态学证据具有较强的一致性，其中包括8个首次测序的物种。本研究还报道了1926年在阿尔及利亚发现的一种新记录，包括两种。此外，分类修订允许将物种dociostus biskrensis Moussi & Petit, 2014转移到窄河马属。分子树分析揭示了新测序物种在Gomphocerinae亚科中的系统发育位置，并为它们的进化关系提供了见解。这些新数据可作为未来在其他地理区域进行研究的起点，使人们能够更好地了解阿尔及利亚这一昆虫群的生物多样性。关键词:BiskraCOI和ITSDNA条形码分子分类新记录窄河马

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来源期刊

African Zoology 生物-动物学

CiteScore

2.60

自引率

9.10%

发文量

审稿时长

>12 weeks

期刊介绍： African Zoology , a peer-reviewed research journal, publishes original scientific contributions and critical reviews that focus principally on African fauna in terrestrial, freshwater, and marine ecosystems. Research from other regions that advances practical and theoretical aspects of zoology will be considered. Rigorous question-driven research in all aspects of zoology will take precedence over descriptive research. The Journal publishes full-length papers, critical reviews, short communications, letters to the editors as well as book reviews. Contributions based on purely observational, descriptive or anecdotal data will not be considered. The Journal is produced by NISC in association with the Zoological Society of South Africa (ZSSA). Acceptance of papers is the responsibility of the Editors-in-Chief in consultation with the Editors and members of the Editorial Advisory Board. All views expressed are those of the author and not necessarily those of the Editors or the Department.