Responses of Phaseolus vulgaris towards Zinc and Iron Management in Soil with respect to Growth, Pigments and Protein Contents

Abstract

An experiment was conducted to study the effect of various zinc amendment levels as well as interactions with iron doses in soil on the growth of plant (length and dry weight), some biochemical parameters such as plant pigments, protein contents, and activity of some enzymes such as catalase and peroxidase in Phaseolus vulgaris (French bean). The amendment of zinc and iron was made as Native soil, 5mg ZnSO4, 25 mg ZnSO4, 50 mg ZnSO4 and 100 mg kg-1 ZnSO4 in soil and their interactive doses with FeSO4 was made as Native soil, 25 mg FeSO4, 25 mg FeSO4 + 25 mg ZnSO4, 50 mg FeSO4 + 5 mg ZnSO4 and 25 mg FeSO4 + 50 mg kg-1 ZnSO4 in soil. The experiment was conducted in triplicates. The maximum value of the dry weight, pigments, and protein content was found at the application of 50 mg kg-1 ZnSO4 + 25 mg kg-1 FeSO4 in soil. The increase in dry weight, total chlorophyll, and protein contents by 48, 44.4, and 37.3 were observed maximum in French bean at the application of 50 mg kg-1 ZnSO4 soil and 25 mg kg-1 FeSO4 in the soil. Maximum tissue accumulation of Fe was found at the high dose of Fe with low Zn-dose, the tissue accumulation showed antagonistic effects of zinc and iron.