Practical Application of the AMRcloud Online Platform for Veterinary Monitoring of Antibiotic Resistance of Zoonotic Pathogens

Abstract

Background . Veterinary monitoring of antibiotic resistance in pathogens of zoonotic diseases is necessary for the rational use of antibiotics. Integration and use of the AMRcloud online platform in veterinary institutions will significantly simplify data processing, increase the speed of data analysis of monitoring results, and help overcome the interagency barrier in the exchange of information between human medicine and veterinary medicine. The aim of the study is the practical ap- plication of the AMRcloud online platform for veterinary monitoring of antibiotic resistance in pathogens of zoonotic origin. Material and methods. In the course of veterinary monitoring, the susceptibility of 275 isolates (16 species of bacteria) to 13 antimicrobial drugs most commonly used in animal husbandry was determined. Among the antimicrobial drugs were: amoxiclav, ampicillin, benzylpenicillin, gentamicin, clindamycin, streptomycin, tetracycline, tobramycin, cephalexin cefotaxime, ceftriaxone, erythromycin, and colistin. The data obtained were combined into a single system using the AMRcloud online platform. Results . The most common causative agents of mastitis were identified: Streptococcus agalactiae (n=96), Escherichia coli (n=55), Staphylococcus aureus (n=33) and Enterococcus faecalis (n=31). ESBL production have been detected in 13 (19.7%) of 66 isolates of the order Enterobacterales, isolated from clinical material of cattle and small ruminants, which limits the use of beta-lactam antibiotics for the treatment of mastitis. Conclusion. The use of the AMRcloud online platform in veterinary laboratories greatly simplifies data processing, increases the result analysis speed, and provides an opportunity to exchange information on antibiotic resistance between laboratories.