Phylogenetic relationships among Australian native citrus species based upon complete chloroplast genomes and single copy nuclear genes

Upuli Nakandala, Agnelo Furtado, Malcolm W. Smith, Darren C. Williams, Robert J. Henry
{"title":"Phylogenetic relationships among Australian native citrus species based upon complete chloroplast genomes and single copy nuclear genes","authors":"Upuli Nakandala, Agnelo Furtado, Malcolm W. Smith, Darren C. Williams, Robert J. Henry","doi":"10.48130/tp-2023-0021","DOIUrl":null,"url":null,"abstract":"Citrus is widely consumed across the world as a fruit crop. Despite many citrus species being extensively studied around the world, phylogenetic relationships among Australian native species remain unresolved. Here we present the phylogenetic relationships among six Australian native species, two domesticated citrus cultivars of commercial importance in Australia, and another 13 accessions cultivated internationally based on complete, <italic>de novo</italic> assembled chloroplast genomes and 86 single copy nuclear genes. The chloroplast and nuclear phylogenies were topologically different. The Australian species formed a monophyletic clade based on their nuclear genes. The nuclear phylogeny revealed a close relationship between <italic>Citrus inodora</italic> and <italic>Citrus australasica.</italic> These two species were distinct from the other four Australian limes that were more closely related to each other. <italic>Citrus australasica</italic> had a unique chloroplast which was distinct from all other Australian limes. Among the other Australian limes, <italic>Citrus glauca</italic> was the most distinct species based on nuclear genes, however, it had a similar chloroplast sequence to <italic>C. australis</italic>. The undetermined <italic>Citrus</italic> sp. was more closely related to <italic>Citrus garrawayi</italic>, indicating that it is a distinct form of <italic>Citrus garrawayi</italic>. <italic>Citrus medica,</italic> had a chloroplast similar to Australian species. However, the nuclear gene phylogeny analysis revealed that <italic>C. medica</italic> was more closely related to Asian citrus species. This study improves our understanding of phylogenetic relationships among Australian citrus species and confirms their unique status within the genus since it formed a monophyletic clade which was clearly separated from the other non-Australian species.","PeriodicalId":120197,"journal":{"name":"Tropical Plants","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plants","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.48130/tp-2023-0021","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Citrus is widely consumed across the world as a fruit crop. Despite many citrus species being extensively studied around the world, phylogenetic relationships among Australian native species remain unresolved. Here we present the phylogenetic relationships among six Australian native species, two domesticated citrus cultivars of commercial importance in Australia, and another 13 accessions cultivated internationally based on complete, de novo assembled chloroplast genomes and 86 single copy nuclear genes. The chloroplast and nuclear phylogenies were topologically different. The Australian species formed a monophyletic clade based on their nuclear genes. The nuclear phylogeny revealed a close relationship between Citrus inodora and Citrus australasica. These two species were distinct from the other four Australian limes that were more closely related to each other. Citrus australasica had a unique chloroplast which was distinct from all other Australian limes. Among the other Australian limes, Citrus glauca was the most distinct species based on nuclear genes, however, it had a similar chloroplast sequence to C. australis. The undetermined Citrus sp. was more closely related to Citrus garrawayi, indicating that it is a distinct form of Citrus garrawayi. Citrus medica, had a chloroplast similar to Australian species. However, the nuclear gene phylogeny analysis revealed that C. medica was more closely related to Asian citrus species. This study improves our understanding of phylogenetic relationships among Australian citrus species and confirms their unique status within the genus since it formed a monophyletic clade which was clearly separated from the other non-Australian species.
基于全叶绿体基因组和单拷贝核基因的澳大利亚本土柑橘种系统发育关系
柑橘作为一种水果作物在世界各地被广泛消费。尽管许多柑橘物种在世界各地被广泛研究,但澳大利亚本土物种之间的系统发育关系仍未得到解决。本文基于完整的、从头组装的叶绿体基因组和86个单拷贝核基因,对6个澳大利亚本土品种、2个在澳大利亚具有重要商业价值的驯化柑橘品种和另外13个国际栽培品种进行了系统发育关系的研究。叶绿体和细胞核系统发育在拓扑结构上存在差异。澳大利亚种根据其核基因形成了一个单系进化枝。核系统发育结果表明,柑橘与澳洲柑橘亲缘关系密切。这两个物种与其他四种澳大利亚酸橙不同,这些酸橙彼此关系更密切。澳大利亚柑橘有一个独特的叶绿体,不同于所有其他澳大利亚酸橙。在其他澳大利亚酸橙中,柑橘(Citrus glauca)是核基因最独特的物种,但其叶绿体序列与澳大利亚酸橙(C. australis)相似。未确定的Citrus sp.与Citrus garrawayi亲缘关系更近,表明它是Citrus garrawayi的一个独特形式。柑桔属植物,叶绿体与澳大利亚种相似。然而,核基因系统发育分析表明,草柑与亚洲柑橘的亲缘关系更近。这项研究提高了我们对澳大利亚柑橘物种之间系统发育关系的理解,并证实了它们在属内的独特地位,因为它形成了一个单系进化枝,与其他非澳大利亚物种明显分开。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信