Antioxidant and Anticancer Activity of Rhynchosia rufescens (Willd.) DC.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.56042/ijbb.v60i11.717

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引用次数: 0

Abstract

The present study deals with the chemical composition, antioxidant, antimicrobial and anticancer efficacy of Rhynchosia rufescens (RR) leaf methanol (RRME), ethyl acetate (RREAE) and petroleum ether (RRPE) extracts. Preliminary phytochemical results revealed that alkaloids, coumarins, flavonoids, volatile oils, fatty acids, and phenols were present in the test extracts. Gas chromatographic analysis of RRPE revealed the identification of 25 volatile compounds. The oil contains monoterpenes (9.6%) and fatty acids (6.12%). The major components of the oil are beta- copaene (32.06%), cyclofenchene (16.53%), beta- pinene (5.69%), 3-carene (3.48%) and sabinene (3.22%). Quantitative estimation of total phenols (TPC) and flavonoids (TFC) indicated that TPC ranged from 8.99 ±1.00 to 60.00±1.15 mg GAE/g dry weight and TFC was between 4.98±0.08 to 20.4±0.50 mg QE/ g dry weight respectively. Methanol extract showed the highest phosphomolybdenum dependent antioxidant capacity, while ethyl acetate extracts strongly inhibited DPPH and hydroxyl radicals. Anticancer study results revealed that ethyl acetate extract enhanced caspase-3 mediated DNA fragmentation in HCT – 116 cell lines than standard cisplatin. In conclusion, R. rufescens extracts showed potent antioxidant, antibacterial activity and potential anticancer activity in HCT-116 cell lines. The observations of this study indicated that R. rufescens extracts are a rich source of natural antioxidants and anticancer components.

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金缕草(野生)的抗氧化和抗癌活性直流。

本文研究了毛茛叶甲醇(RRME)、乙酸乙酯(RREAE)和石油醚(RRPE)提取物的化学成分、抗氧化、抑菌和抗癌作用。初步植物化学结果显示，试验提取物中含有生物碱、香豆素、类黄酮、挥发油、脂肪酸和酚类物质。RRPE气相色谱分析鉴定出25种挥发性化合物。这种油含有单萜烯(9.6%)和脂肪酸(6.12%)。主要成分为- - copaene(32.06%)、环番烯(16.53%)、- -蒎烯(5.69%)、3-蒈烯(3.48%)和sabinene(3.22%)。总酚(TPC)和总黄酮(TFC)含量测定结果表明，总酚(TPC)含量在8.99±1.00 ~ 60.00±1.15 mg QE/ g干重之间，总黄酮含量在4.98±0.08 ~ 20.4±0.50 mg QE/ g干重之间。甲醇提取物对磷钼依赖的抗氧化能力最强，而乙酸乙酯提取物对DPPH和羟基自由基的抑制作用最强。抗癌研究结果显示，与标准顺铂相比，乙酸乙酯提取物可增强HCT - 116细胞株中caspase-3介导的DNA断裂。综上所述，红草提取物对HCT-116细胞株具有较强的抗氧化、抗菌活性和潜在的抗癌活性。本研究结果表明，红皮草提取物具有丰富的天然抗氧化剂和抗癌成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.