Preliminary assessment of the safety of genetically modified food products

Tvarinnictvo ta tehnologìï harčovih produktìv Pub Date : 2023-07-28 DOI:10.31548/animal.3.2023.9

Yuriy Balji

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引用次数: 1

Abstract

Numerous studies on genetically modified food products indicate their safety and their potential to alleviate hunger worldwide. However, the issue of GM products and feeds remains relevant in the realm of food security. The purpose of this study is to present a comprehensive scheme of methods for determining the safety of GM food products and to develop a new method for assessing the safety of GM food products. This new method is based on the use of a biotest and the employment of Paramecium caudatum infusoria. In the study, GTS 40-3 and GTS 40-3-2 (Venus variety) soybeans, MON 00603 (NK 603) maize line, and non-GMO soybeans and maize were used as controls. Infusoria were prepared by culturing them in room temperature water (18- 23°C) without direct sunlight, with the addition of non-GMO yeast powder as a nutrient solution and senna extract. Subsequently, the initial material containing approximately 100 infusoria was transferred to 100 ml flasks and incubated for 2-3 days. At the initial stage of the experiment, from day 1 to day 15, daily records and visual assessments were conducted. The assessment included the quantity of infusoria, motility (chemotaxis), size, shape, and the percentage of dead infusoria. Venus soybeans (GTS 40-3-2) exhibited a toxic effect on the protozoa, which died in repeated trials on the 14th-15th day of feeding. It was observed that infusoria fed with soybeans containing the GTS 40-3-2 (Venus) transformation event changed their shape after 3 days from the start of the experiment. Over the course of 10 days from the beginning of the study, a decrease in movement, motility, and the quantity of infusoria was noted. On the 14th-15th day, mortality was observed. Negative changes were also observed in infusoria that were fed with MON 00603 (NK 603) maize. The application of this method in the practice of assessing the safety of GM food products can provide a preliminary evaluation of long-term effects over a relatively short period

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转基因食品安全性初步评估

关于转基因食品的大量研究表明，转基因食品的安全性及其在缓解全球饥饿方面的潜力。然而，转基因产品和饲料问题在粮食安全领域仍然具有相关性。本研究的目的是提出一套确定转基因食品安全性的综合方法方案，并开发一种评估转基因食品安全性的新方法。这种新方法是基于生物试验的使用和尾状草履虫的使用。以GTS 40-3和GTS 40-3-2(金星品种)大豆、MON 00603 (NK 603)玉米品系、非转基因大豆和玉米为对照。在室温(18 ~ 23℃)无光照条件下，加入非转基因酵母粉作为营养液，添加番泻叶提取物，培养绒毛菌。随后，将含有约100个虫体的初始材料转移到100 ml烧瓶中，孵育2-3天。试验初期(第1 ~ 15天)进行日常记录和目测评价。评价指标包括虫体数量、运动性(趋化性)、大小、形状和死亡虫体百分比。金星大豆(GTS 40-3-2)在饲养第14 ~ 15天的重复试验中表现出对原生动物的毒杀作用。试验开始后3 d，经GTS 40-3-2(金星)转化事件的大豆饲养的瓢虫形状发生变化。在研究开始后的10天内，观察到运动、运动性和滴虫数量的减少。第14 ~ 15天观察死亡率。饲喂MON 00603 (NK 603)玉米的棉铃虫也出现了阴性变化。在评估转基因食品安全性的实践中应用这种方法可以在相对较短的时间内对长期影响进行初步评估

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Tvarinnictvo ta tehnologìï harčovih produktìv

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