{"title":"Exogenous enzyme addition affects litter decomposition by altering the microbial community and litter nutrient content in planted forest","authors":"Huayue Nie, Chenrui Wang, Meirong Tian, Jixi Gao","doi":"10.1093/jpe/rtad031","DOIUrl":null,"url":null,"abstract":"Abstract Litter inputs greatly impact the soil properties and ecosystem functioning in forests. Quick litter decomposition is promoted, which decreases planted forest agricultural waste and increases the nutrient cycle in forests. The breakdown of litter and the release of various components depend heavily on enzymes. However, the effects of exogenous enzyme preparations on litter decomposition have hardly ever been researched. In this study, we examine how these enzymes affect the remaining rate of litter quality, nutrient content( C, N, K ), and microbial community diversity. Taking artificial Eriobotrya japonica litter as the research object, applied five exogenous enzymes (laccase, lignin peroxidase, leucine arylamidase, cellulase, and acid phosphatase) to litter leaves. During the decomposition period, the mass remaining rate and main nutrient content of the litter were measured. After decomposition at constant temperature and humidity for 189 days, determine the microbial diversity attached to the surface of the litter. Laccase and lignin peroxidase increase litter degradation by affecting microbial diversity, nitrogen (N), and kalium (K) contents. Leucine arylamidase leads to an enrichment of N, which decreases the quality of the litter. The cellulose and lignin decomposition rate in litters was unaffected by the addition of cellulase, laccase, and lignin peroxidase. Our analysis emphasizes that exogenous enzymes may alter the nutrient content and microbial community, which may affect litter decomposition. To regulate the rate at which agricultural waste litter decomposes, it is imperative to investigate the effect and mechanism of exogenous enzymes on litter decomposition.","PeriodicalId":50085,"journal":{"name":"Journal of Plant Ecology","volume":"330 1","pages":"0"},"PeriodicalIF":3.0000,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Ecology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/jpe/rtad031","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract Litter inputs greatly impact the soil properties and ecosystem functioning in forests. Quick litter decomposition is promoted, which decreases planted forest agricultural waste and increases the nutrient cycle in forests. The breakdown of litter and the release of various components depend heavily on enzymes. However, the effects of exogenous enzyme preparations on litter decomposition have hardly ever been researched. In this study, we examine how these enzymes affect the remaining rate of litter quality, nutrient content( C, N, K ), and microbial community diversity. Taking artificial Eriobotrya japonica litter as the research object, applied five exogenous enzymes (laccase, lignin peroxidase, leucine arylamidase, cellulase, and acid phosphatase) to litter leaves. During the decomposition period, the mass remaining rate and main nutrient content of the litter were measured. After decomposition at constant temperature and humidity for 189 days, determine the microbial diversity attached to the surface of the litter. Laccase and lignin peroxidase increase litter degradation by affecting microbial diversity, nitrogen (N), and kalium (K) contents. Leucine arylamidase leads to an enrichment of N, which decreases the quality of the litter. The cellulose and lignin decomposition rate in litters was unaffected by the addition of cellulase, laccase, and lignin peroxidase. Our analysis emphasizes that exogenous enzymes may alter the nutrient content and microbial community, which may affect litter decomposition. To regulate the rate at which agricultural waste litter decomposes, it is imperative to investigate the effect and mechanism of exogenous enzymes on litter decomposition.
凋落物输入对森林土壤性质和生态系统功能有很大影响。促进凋落物快速分解,减少人工林农业废弃物,增加森林养分循环。凋落物的分解和各种成分的释放在很大程度上依赖于酶。然而,外源酶制剂对凋落物分解的影响鲜有研究。在这项研究中,我们研究了这些酶如何影响凋落物质量的剩余率,营养成分(C, N, K)和微生物群落多样性。以人工枇杷凋落叶为研究对象,将5种外源酶(漆酶、木质素过氧化物酶、亮氨酸芳基酰胺酶、纤维素酶、酸性磷酸酶)应用于凋落叶。在分解过程中,测定凋落物的质量剩余率和主要养分含量。恒温恒湿分解189天后,测定凋落物表面附着的微生物多样性。漆酶和木质素过氧化物酶通过影响微生物多样性、氮和钾含量来促进凋落物的降解。亮氨酸芳酰胺酶导致N的富集,从而降低凋落物的质量。添加纤维素酶、漆酶和木质素过氧化物酶对凋落物中纤维素和木质素的分解速率没有影响。我们的分析强调,外源酶可能改变养分含量和微生物群落,从而影响凋落物的分解。为了调控农业废弃物凋落物的分解速率,研究外源酶对凋落物分解的影响及其机制势在必行。
期刊介绍:
Journal of Plant Ecology (JPE) serves as an important medium for ecologists to present research findings and discuss challenging issues in the broad field of plants and their interactions with biotic and abiotic environment. The JPE will cover all aspects of plant ecology, including plant ecophysiology, population ecology, community ecology, ecosystem ecology and landscape ecology as well as conservation ecology, evolutionary ecology, and theoretical ecology.