Investigation of mucus cell change in gills of van fish ( Alburnus tarichi , Güldenstädt, 1814) treated with fluvalinate

Abstract

ABSTRACTThe Van fish is an endemic carp species living in Lake Van, one of the largest soda lakes in the world. Fluvalinate is a pesticide used extensively in the Lake Van basin. In the present study, the effects of sublethally applied fluvalinate on mucous cells in Van fish gill tissue after 48, 72, and 96 h were determined histochemically. Histopathological lesions were observed in the gills of the fish exposed to fluvalinate in a time-dependent manner. The concentrations of neutral glycoconjugates, acidic glycoconjugates, sulfated acidic glycoconjugates, and strongly acidic glycoconjugates in the mucous cells increased in the gills of the Van fish during the exposure period. At the same time, the area and number of mucous cells increased during fluvalinate exposure. As a result, fluvalinate caused an increase in mucous secretion and content in Van fish gills.KEYWORDS: Fluvalinateglycoconjugatehistopathologypesticideteleost Disclosure statementNo potential conflict of interest was reported by the authors.Ethics approvalAll of the animal experimental procedures were performed in accordance with the animal study protocols approved by the Animal Researchers Local Ethic Committee of Van Yüzüncü Yıl University (protocol no: 2018/05) and the Republic of Turkey Ministry of Agriculture and Forestry (31/05/2018).Availability of data and materialAll relevant data are within the paper, and those are available at the corresponding author.Authors’ contributionsAhmet Regaib Oğuz and Elif Kaval Oğuz conceived the study and designed the experiments. Elif Kaval Oğuz and Zehra Alkan carried out the histology analysed the data. Ahmet Regaib Oğuz and Elif Kaval Oğuz wrote and revised the manuscript. All authors read and approved the final manuscript.Additional informationNotes on contributorsZehra AlkanZehra Alkan completed her doctorate at Van Yüzüncü Yıl University. His studies focused on fish physiology.Elif Kaval OğuzElif Kaval Oğuz is an associate professor in the Department of Science Education, Van Yüzüncü Yıl University, Turkey. She obtained her PhD in Neuroscience from Medicine Faculty. She is currently working with cell cultures.Ahmet Regaib OğuzAhmet Regaib Oğuz is a professor in the Department of Biology, Van Yüzüncü Yıl University, Turkey. His research focuses on ecotoxicity and osmoregulation.