{"title":"Genetic Diversity of Common Wild Wheat Species in Armenia Using ISSR Markers","authors":"Manvel Badalyan, Tatevik Aloyan, Varya Dilanyan, Satenik Kharatyan, Aghvan Sahakyan, Andreas Melikyan","doi":"10.3844/ojbsci.2023.389.401","DOIUrl":null,"url":null,"abstract":"Armenia is one of the primary genealogical centers of numerous crops, the wild relatives of many of which are still found in Armenia today. Population limits of three out of 4 species of wild relatives of endemic wheat in Armenia (T. araraticum Jakubz, T. urartu Tumanian ex Gandilyan, T. boeoticum Boiss.) have been reduced in recent decades as a result of various ecological and economic functions, they are facing the danger of extinction and are registered in the Red Book of Plants of the Republic of Armenia. In order to identify these species as selection raw material, genetic characterization of populations was carried out according to ISSR-PCR markers, as well as decoding genetic formulas and barcodes. As a result, according to the coefficients of the genetic pattern, the studied wild types of wheat are characterized as basic or typical gene pools with the least quantity and frequency of rare alleles. The populations are stable and in conditions of efficient number of isolates, the gene pool is capable of self-reproduction. The obtained results as genetic markers will provide an opportunity to further clarify the origin of the species, evolution, patterns of inheritance of useful economic traits, cell genetic characteristics of the species, natural resistance to adverse environmental factors and diseases, etc.","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":"23 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"OnLine Journal of Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3844/ojbsci.2023.389.401","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
Armenia is one of the primary genealogical centers of numerous crops, the wild relatives of many of which are still found in Armenia today. Population limits of three out of 4 species of wild relatives of endemic wheat in Armenia (T. araraticum Jakubz, T. urartu Tumanian ex Gandilyan, T. boeoticum Boiss.) have been reduced in recent decades as a result of various ecological and economic functions, they are facing the danger of extinction and are registered in the Red Book of Plants of the Republic of Armenia. In order to identify these species as selection raw material, genetic characterization of populations was carried out according to ISSR-PCR markers, as well as decoding genetic formulas and barcodes. As a result, according to the coefficients of the genetic pattern, the studied wild types of wheat are characterized as basic or typical gene pools with the least quantity and frequency of rare alleles. The populations are stable and in conditions of efficient number of isolates, the gene pool is capable of self-reproduction. The obtained results as genetic markers will provide an opportunity to further clarify the origin of the species, evolution, patterns of inheritance of useful economic traits, cell genetic characteristics of the species, natural resistance to adverse environmental factors and diseases, etc.
亚美尼亚是许多作物的主要谱系中心之一,其中许多作物的野生近亲今天仍然在亚美尼亚被发现。近几十年来,亚美尼亚特有小麦的4种野生近缘种(T. araratium Jakubz, T. urartu Tumanian ex Gandilyan, T. boeoticum Boiss.)中的3种(T. araratium Jakubz, T. urartu Tumanian ex Gandilyan, T. boeoticum Boiss.)由于各种生态和经济功能,种群限制已经减少,它们面临灭绝的危险,并被登记在亚美尼亚共和国植物红皮书中。为了确定这些物种作为选择原料,根据ISSR-PCR标记对群体进行遗传鉴定,并对遗传公式和条形码进行解码。结果表明,在所研究的小麦野生型中,稀有等位基因数量最少、频率最低的是基本基因库或典型基因库。种群稳定,在有效分离数量的条件下,基因库能够自我繁殖。作为遗传标记所获得的结果将为进一步阐明物种的起源、进化、有用经济性状的遗传模式、物种的细胞遗传特征、对不利环境因素和疾病的自然抗性等提供机会。