The structure of some blood-borne infections distribution among persons from penitentiary institutions the markers

Abstract

The aim of the study was to assess the occurrence of serological and molecular genetic markers of HIV infection, viral hepatitis B and C among persons in pre-trial detention. Materials and methods. The material of the study identified 138 samples of blood sequences obtained from men in the pretrial detention center in St. Petersburg. Patients were examined by ELISA for the presence of serological markers of HIV infection (Ag/Ab HIV), HBV (HBsAg, antiHBs IgG, anti-HBcore IgG) and HCV (anti-HCV IgG). Determination of HIV and HCV RNA, as well as HBV DNA by PCR with hybridization-fluorescence detection in «real time» mode. HBV DNA with a low viral form was detected using a technique developed at the Saint-Petersburg Pasteur Institute. Results and discussion . With the prevalence of the general prevalence of serological markers, markers of HIV infection were detected in 55 (39.86%) patients, HBV — in 73 (52.90%), HCV — in 83 (60.14%). the same markers of HIV infection and HBV were detected in 6 (4.35%) examined, HIV infection and HCV in 17 (12.32%), HBV and HCV in 22 (15.94%) persons, to all three infections in 26 (18.84%) cases. In 22 (15.94%) people did not notice any of the analyzed markers. HIV RNA was found in 23.19%, HCV RNA — in 46.38%, HBV DNA — in 10.14% of the examined. At the same time, HBV DNA with a low viral form (less than 50 IU/ml) was detected in 7.97% of cases, including 6.52% of seronegative samples. Simultaneously, HIV and HCV RNA were detected in 6.52%, HCV RNA and HBV DNA in 2.17% of patients. Molecular biological markers of all three indicators were found in 1.45% of patients. The examined group was ranked by age into the following subgroups: 21– 30 years old (12.32%), 31–40 years old (34.78%), 41–50 years old (37.68%) and older than 51 years old (15.22%). Certain differences in the prevalence and distribution of the analysis of serological and molecular biological markers between age markers, the highest frequency was found for groups of 31–40 and 41–50 years. Conclusion. The results obtained indicate a high prevalence of HIV infections and parenteral viral hepatitis B and C among persons convicted and awaiting a stage in a pre-trial detention center. Careful attention must be paid to their admission to penitentiary institutions, since many convicts are not aware of their morbidity and may become infected. Regular conclusions, concluded in dynamics, including laboratory diagnostics using highly sensitive molecular biological methods, upon admission to a penitentiary institution, and then annually and upon release, should be taken into account as measures to prevent infection with HIV, HBV, HCV.