Co-culture models for investigating cellular crosstalk in the glioma microenvironment

Cancer pathogenesis and therapy Pub Date : 2023-11-07 DOI:10.1016/j.cpt.2023.11.002

{"title":"Co-culture models for investigating cellular crosstalk in the glioma microenvironment","authors":"","doi":"10.1016/j.cpt.2023.11.002","DOIUrl":null,"url":null,"abstract":"<div><div>Glioma is the most prevalent primary malignant tumor in the central nervous system (CNS). It represents a diverse group of brain malignancies characterized by the presence of various cancer cell types as well as an array of noncancerous cells, which together form the intricate glioma tumor microenvironment (TME). Understanding the interactions between glioma cells/glioma stem cells (GSCs) and these noncancerous cells is crucial for exploring the pathogenesis and development of glioma. To invesigate these interactions requires <em>in vitro</em> co-culture models that closely mirror the actual TME <em>in vivo</em>. In this review, we summarize the two- and three-dimensional <em>in vitro</em> co-culture model systems for glioma-TME interactions currently available. Furthermore, we explore common glioma-TME cell interactions based on these models, including interactions of glioma cells/GSCs with endothelial cells/pericytes, microglia/macrophages, T cells, astrocytes, neurons, or other multi-cellular interactions. Together, this review provides an update on the glioma-TME interactions, offering insights into glioma pathogenesis.</div></div>","PeriodicalId":93920,"journal":{"name":"Cancer pathogenesis and therapy","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949713223000915/pdfft?md5=4f95c308ede234e85c3fe23111757339&pid=1-s2.0-S2949713223000915-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cancer pathogenesis and therapy","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2949713223000915","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Glioma is the most prevalent primary malignant tumor in the central nervous system (CNS). It represents a diverse group of brain malignancies characterized by the presence of various cancer cell types as well as an array of noncancerous cells, which together form the intricate glioma tumor microenvironment (TME). Understanding the interactions between glioma cells/glioma stem cells (GSCs) and these noncancerous cells is crucial for exploring the pathogenesis and development of glioma. To invesigate these interactions requires in vitro co-culture models that closely mirror the actual TME in vivo. In this review, we summarize the two- and three-dimensional in vitro co-culture model systems for glioma-TME interactions currently available. Furthermore, we explore common glioma-TME cell interactions based on these models, including interactions of glioma cells/GSCs with endothelial cells/pericytes, microglia/macrophages, T cells, astrocytes, neurons, or other multi-cellular interactions. Together, this review provides an update on the glioma-TME interactions, offering insights into glioma pathogenesis.

Abstract Image

查看原文本刊更多论文

用于研究胶质瘤微环境中细胞串联的共培养模型

胶质瘤是中枢神经系统（CNS）中最常见的原发性恶性肿瘤。胶质瘤是一种多样化的脑恶性肿瘤，其特点是存在各种癌细胞类型以及一系列非癌细胞，它们共同构成了错综复杂的胶质瘤肿瘤微环境（TME）。了解胶质瘤细胞/胶质瘤干细胞（GSCs）与这些非癌细胞之间的相互作用对于探索胶质瘤的发病机制和发展至关重要。要研究这些相互作用，需要建立与体内实际TME密切相关的体外共培养模型。在这篇综述中，我们总结了目前可用的胶质瘤-TME相互作用的二维和三维体外共培养模型系统。此外，我们还根据这些模型探讨了常见的胶质瘤-TME细胞相互作用，包括胶质瘤细胞/间充质干细胞与内皮细胞/水肿细胞、小胶质细胞/巨噬细胞、T细胞、星形胶质细胞、神经元的相互作用或其他多细胞相互作用。综上所述，本综述提供了胶质瘤-TME相互作用的最新情况，为胶质瘤的发病机制提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cancer pathogenesis and therapy Surgery, Radiology and Imaging, Cancer Research, Oncology

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

54 days