Phasor Identifier: A Cloud-based Analysis of Phasor-FLIM Data on Python Notebooks

Abstract

This paper introduces an innovative approach utilizing Google Colaboratory (Colab) for the versatile analysis of phasor Fluorescence Lifetime Imaging Microscopy (FLIM) data collected from various samples (e.g., cuvette, cells, tissues) and in various input file formats. In fact, phasor-FLIM widespread adoption has been hampered by complex instrumentation and data analysis requirements. We mean to make advanced FLIM analysis more accessible to researchers through a cloud-based solution that i) harnesses robust computational resources, ii) eliminates hardware limitations, iii) supports both CPU and GPU processing, We envision a paradigm shift in FLIM data accessibility and potential, aligning with the evolving field of AI-driven FLIM analysis. This approach simplifies FLIM data handling and opens doors for diverse applications, from studying cellular metabolism to investigating drug encapsulation, benefiting researchers across multiple domains. The comparative analysis of freely distributed FLIM tools highlights the unique advantages of this approach in terms of adaptability, scalability, and open-source nature.