Spectral Reflectance Estimated Genetic Variation in Hybrid Turf Bermudagrass

Desalegn D. Serba, Yanqi Wu, Reagan Hejl, Clinton Williams, Kevin Bronson
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Abstract

High throughput phenotyping (HTP) utilizing both remote and proximal sensing technologies has emerged as a vital tool for evaluating the biophysical characteristics of turfgrass. This study was conducted to assess the genetic diversity of hybrid turf bermudagrass using spectral reflectance indices and use of HTP for germplasm enhancement. A total of 50 accessions of the hybrid bermudagrass (Cynodon dactylon × C. transvaalensis) were grown in the greenhouse in three replications. The spectral data were gathered using a height independent active crop canopy sensor, 'RapidScan CS-45', which measures canopy reflectance at the wavelengths of 670 nm, 730 nm, and 780 nm. The reflectance data were used to derive three indices related to canopy photosynthetic area and other three related to chlorophyll content. All vegetation indices showed significant genotype-to-genotype variation. Ten superior genotypes were identified using the multi-trait genotype-ideotype distance index (MGIDI) as a selection differential. On 48 of the genotypes that were established in the field in two replications, establishment rate and winter color data were also gathered. The results of a linear regression analysis demonstrated the importance of spectral vegetation indices (SVI) for the turfgrass quick establishment (percentage area coverage) and winter color retention. This study brings attention to the potential use of the proximal sensing in turfgrass germplasm enhancement for establishment speed, aesthetic value, and mild-winter color retention.
杂交草坪百慕大草的光谱反射率估计遗传变异
利用遥感和近端传感技术的高通量表型(HTP)已成为评估草坪草生物物理特性的重要工具。利用光谱反射率指数和HTP技术对杂交草坪百慕大草的遗传多样性进行了评价。在温室中分3个重复种植50份杂交百米草(Cynodon dactylon × C. transvaalensis)。光谱数据的收集使用高度无关的作物冠层主动传感器RapidScan CS-45,该传感器测量670 nm、730 nm和780 nm波长的冠层反射率。利用反射率数据推导出与冠层光合面积相关的3个指标和与叶绿素含量相关的3个指标。所有植被指数均表现出显著的基因型差异。利用多性状基因型-理想型距离指数(MGIDI)作为选择差异,鉴定出10个优势基因型。对田间建立的48个基因型进行了2个重复的建立率和冬色数据的收集。线性回归分析结果表明,光谱植被指数(SVI)对草坪草快速建立(面积覆盖率百分比)和冬季颜色保持具有重要意义。本研究将关注近端感应在草坪草种质增强中的潜在应用,包括建立速度、美学价值和暖冬颜色保持。
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