Genome-wide identification and expression pattern analysis of the ACS gene family during fruit development in peach

Q4 Agricultural and Biological Sciences

Fruit Growing Research Pub Date : 2023-01-01 DOI:10.48130/frures-0023-0040

Xiaofei Wang, Kang Dong, Jun Cheng, Bin Tan, Xianbo Zheng, Xia Ye, Wei Wang, Langlang Zhang, Jiancan Feng

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引用次数: 0

Abstract

Ethylene plays an important role in regulating the development and ripening of fruits, and 1-aminocyclopropane carboxylic acid synthase is the key rate-limiting enzyme in ethylene synthesis pathway. In this study, eight PpACS genes were identified from the peach genome [Prunus persica (L.) Batsch], and their phylogeny, gene structures, promoter motifs and expression patterns were analyzed. The PpACS genes could be divided into four types, and the genes with similar structures and motif distribution clustered together. Identification of the cis-elements in the promoters revealed that the PpACS genes may respond to various hormones. Furthermore, expression analysis showed that five (PpACS1, PpACS5, PpACS6, PpACS7 and PpACS8) of the eight PpACS genes were expressed at different stages during peach fruit development. Among them, PpACS1 was highly expressed at the ripening stage and induced by ethylene. The expression peaks of PpACS5, PpACS6, PpACS7 and PpACS8 during the transition from first exponential growth to pit hardening (S1 to S2) indicated a potential function of ethylene during this important transition. Taken together, these results provide valuable information for future investigation into the functions of the PpACS genes during peach fruit development and ripening.

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ACS全基因组鉴定及表达模式分析桃果实发育过程中的基因家族

乙烯在调节果实发育和成熟中起着重要作用，而1-氨基环丙烷羧酸合成酶是乙烯合成途径中关键的限速酶。本研究从桃基因组中鉴定出8个PpACS基因[Prunus persica (L.)]。对它们的系统发育、基因结构、启动子基序和表达模式进行了分析。PpACS基因可分为四种类型，具有相似结构和基序分布的基因聚集在一起。启动子中顺式元件的鉴定表明PpACS基因可能对多种激素有反应。此外，表达分析表明，8个PpACS基因中的5个(PpACS1、PpACS5、PpACS6、PpACS7和PpACS8)在桃果实发育的不同阶段均有表达。其中，PpACS1在催熟期高表达，受乙烯诱导。PpACS5、PpACS6、PpACS7和PpACS8在第一次指数生长到坑硬化阶段(S1到S2)的表达峰表明乙烯在这一重要转变过程中发挥了潜在的作用。这些结果为进一步研究PpACS基因在桃果发育和成熟过程中的功能提供了有价值的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fruit Growing Research Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

0.10

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： FRUIT GROWING RESEARCH is an international journal, which has been issued under the care of the Research Institute for Fruit Growing Pitesti, Romania. Fruit growing research is an annual journal aimed at disseminating significant research and original papers on genetic resources, breeding of deciduous fruit trees species; fruit sustainable technologies – environment protection; propagation, virology and tissue culture, postharvest, that can be of interest to a wide audience of plant scientists in all areas of fruit growing. An international Editorial Board advises the journal.

Genome-wide identification and expression pattern analysis of the <i>ACS</i> gene family during fruit development in peach

Abstract