Effect of herbicides on cellular growth, microbiological and physiological activities of an identified rhizosphere isolate Rhodococcus erythropolis

IF 1.3 4区 环境科学与生态学 Q3 ECOLOGY
Mohammad Shahid, Mohammad Saghir Khan, Asad Syed, Abdallah M. Elgorban, John Pichtel, Ling Shing Wong
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Herbicide-induced morphological changes viz. aberrant margins; cellular cracking and distortion/damage in R. erythropolis cells were apparent under SEM observation. Furthermore, herbicide-treated and DAPI (4’,6-diamidino-2-phenylindole)-stained cells showed concentration-dependent reduction in cellular permeability as revealed under CLSM. Furthermore, herbicides displayed toxicity towards bioactive molecules of PSB-6 in a dose-related manner. Among them, ATZ imparted maximum negative effect, where it reduced the bacterial production of IAA, ACC deaminase and 2, 3-DHBA by 68% (p ≤ 0.001), 75% (p ≤ 0.001), and 83% (p ≤ 0.005), respectively, over control. Additionally, following herbicide exposure, bacterial counts (log10 CFU mL−1) were reduced. Higher concentrations of ATZ and BCR completely reduced the growth patten of strain PSB-6. The current investigation provides an insight into a mechanistic approach of chemical herbicide-induced hazard toward a beneficial soil isolate. Careful monitoring is therefore necessary before agricultural application of pesticides.KEYWORDS: Pesticides stressRhodococcus erythropolisEnzymatic profileBacterial activityCellular viabilityActive biomolecules AcknowledgementsThe authors extend their appreciation to the Researchers Supporting Project number (RSP2023R367), King Saud University, Saudi Arabia.Disclosure statementNo potential conflict of interest was reported by the author(s).Author contributionsConceptualisation, M.S. and M.S.K,; methodology, M.S.; software, M.S., A.S.; validation, M.S., M.S.K and A.S; formal analysis, M.S and M.S.K., and A.M.E.; investigation, M.S. and M.S.K; resources, M.S., M.S.K., A.S and A.M.E.; data curation, M.S.; writing – original draft preparation, M.S., and M.S.K; writing – review and editing, M.S., J.P., and M.S.K.; visualisation, M.S.K., and J.P.; supervision, M.S.K.; project administration, M.S. and M.S.K; funding acquisition, A.S., A.M.E., and L.S.W. All authors have read and agreed to the published version of the manuscript.Additional informationFundingThis research was funded by the Researchers Supporting Project number [RSP2023R367], King Saud University, Saudi Arabia.Notes on contributorsMohammad ShahidMohammad Shahid is a postdoctoral fellow at ICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM), Mau, U.P, India. He obtained his PhD (Agricultural Microbiology) from Aligarh Muslim University, Aligarh, India. He has published a large number of papers including research and review on microbiology, plant-microbe interaction, rhizosphere biology and pesticide toxicology in various reputed journals. He has attended several national and international conferences, seminars, and workshops. He is reviewer of several national and international journals.Mohammad Saghir KhanMohammad Saghir Khan is a full Professor at the Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, India. Dr. Khan received his Ph.D. (Microbiology) degree from Govind Ballabh Pant University of Agriculture & Technology, Pant Nagar, India. He has been teaching Microbiology to post-graduate students for the last 25 years and has research experience of 29 years. In addition to his teaching, Dr. Khan is engaged in guiding students for their doctoral degree in Microbiology and has successfully supervised 12 research scholars for Ph.D. degree in Agricultural Microbiology while five research scholars are working under his supervision. He has published over 200 scientific papers with a total citation of 17484 (google citation; h- index 55; i10 index 240) including, original research articles, review articles and book chapters in various national and international publication media. Some of the notable journals in which Dr. Khan has published his work include International Journal of Nanomedicines, Chemosphere, Ecotoxicology, Environmental Pollution, Environmental Chemistry letters, Scientific Reports, Current Microbiology, Microbiological Research, Crop Protection, Pesticide Biochemistry & Physiology, ACS Omega, Food and Chemical Toxicology, European Journal of Agronomy, Archives of Environmental Contamination & Toxicology etc. Dr. Khan has edited eleven books published by the leading international publishers. Of these books, a total of 176535 book chapters have been downloaded worldwide. Dr. Khan has also completed research projects funded by UGC and ICAR. His major research area includes Ecotoxicology, Nanotoxicology, Bioremediation, Environmental Microbiology, Rhizobiology and Immunology. Dr. Khan has also served in different administrative capacities in the university.Asad SyedAsad Syed is an Associate Professor at Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia. He has been awarded Doctorate from the University of Pune, whereas he has completed his research work from CSIR-National Chemical Laboratory, Pune, India, where he served as Research Associate and Senior Research Fellow in the Biochemical Sciences Division. His research interest is in fabrication of nanomaterials, by utilizing fungi, bacteria, plant extracts, protein, etc. Another area of his expertise is secondary metabolite from endophytic fungi. Now he ventured into the potential application of nanomaterials in environment and healthcare. He is serving as reviewer for international peer journals that include materials science and microbiology journals. He is teaching basic microbiology and laboratory skills to bachelor's students and involved in mentoring master's and PhD students at Botany and Microbiology Department, KSU, Riyadh. He has published his findings in international peer-reviewed materials science and microbiology journals.Abdallah M. ElgorbanAbdallah M. Elgorban is an Associate Professor at Centre of Excellence in Biotechnology Research (CEBR), King Saud University, Riyadh, Saudi Arabia from March 2009 till date, with 24 years of research and academic experience. He has published 320 international research papers in peer reviewed international journal, with high impact factors such as 24.3 (Fungal Diversity, Top%1), and also having good impact on Publon as 28 h-index and 4500 citations for published papers. His research interest falls in the areas as Nanotechnology, Microbiology, Biotechnology and Molecular Biology. He has an experience and keen interest to learn and explore varied techniques from biotechnology and nanotechnology.John PichtelJohn Pichtel PhD, CHMM, is a professor in Environment, Geology, and Natural Resources, Ball State University, Muncie, IN 47306, US. Prof. Pichtel received his Ph. D in Agronomy/ Environmental Science from the Ohio State University, Columbus, OH in 1987. He did his programme in Agronomy/Soil Chemistry from the Ohio State University, Columbus, OH in 1983. He completed his BS degree in Natural Resource Management from Rutgers University, New Brunswick, NJ. 1980. His research embraces management of hazardous materials, remediation of contaminated sites, and environmental chemistry. His research group is studying the behaviour of metallic nanoparticles in soil and aquatic environments. He is particularly interested in assessing shifts in microbial communities and changes in plant physiological processes as a result of interaction with nanoparticles. They have evaluated soil chemical properties and plant response during restoration of brownfields and Superfund sites in the United States as well as highly contaminated environs in the United Kingdom, Ireland, Finland, and Thailand. Other research involves investigation of microbially-catalysed decomposition of PCBs, explosives, propellants, and other xenobiotics.Ling Shing WongLing Shing Wong is graduated from The National University of Malaysia (UKM), Bangi, Selangor, Malaysia in 2010 with Ph.D. in Environmental Science, specialized in the development of biosensor for environmental toxicity assessment. He is currently a professor in INTI International University, Nilai, Negeri Sembilan, Malaysia. His research interests include development of biosensor for environmental application, discovery of new reporter groups for bioindicator, as well as algae and cyanobacteria culture technology. He has authored and co-authored more than 130 scientific articles and extends his service as editorial member in many international journals. Dr. Wong is a senior member of Asia-Pacific Chemical, Biological & Environmental Engineering Society (APCBEES) and the Institute of Research Engineers & Doctors (theIRED), Member of Persatuan Sains Analisis Malaysia and Persatuan Pembangunan Sensor Malaysia. He won several innovation awards including a bronze medal from Malaysia Technology Expo (MTE), Kuala Lumpur, Malaysia in 2006, a gold medal from International Invention, Innovation & Technology Exhibition (ITEX), Kuala Lumpur, Malaysia in 2008, a gold medal from Product Design Competition (PRODEC) in 2013, a silver medal from International Innovation Festival (Innofest) in 2014, finalist of National Outstanding Innovator Award in 2015, a silver medal from Private Higher Education Invention and Innovation Contest (PERINTIS) in 2016, a silver medal from International Invention & Innovation Competition in 2017, two bronze medal from International Invention, Innovation and Design Expo (INNODEX) in 2021, and a silver medal from Malaysia Technology Expo (MTE) in 2021.","PeriodicalId":9960,"journal":{"name":"Chemistry and Ecology","volume":"163 1","pages":"0"},"PeriodicalIF":1.3000,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry and Ecology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/02757540.2023.2263436","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

ABSTRACTConsidering the excessive pesticide pollution in the environment, atrazine (ATZ), butachlor (BCR) and quizalofop-p-ethyl (QUIZ) were selected to assess their toxic effect on Rhodococcus erythropolis PSB-6 (NCBI Accession No. MG028649). MIC values of ATZ, BCR and QUIZ to R. erythropolis were determined to be 100, 200 and 150 μM, respectively. Biomarker enzymatic assays including LPO, LDH and oxidative stress (CAT) induced by herbicides represented significant (p ≤ 0.005) toxicity towards strain PSB-6. Herbicide-induced morphological changes viz. aberrant margins; cellular cracking and distortion/damage in R. erythropolis cells were apparent under SEM observation. Furthermore, herbicide-treated and DAPI (4’,6-diamidino-2-phenylindole)-stained cells showed concentration-dependent reduction in cellular permeability as revealed under CLSM. Furthermore, herbicides displayed toxicity towards bioactive molecules of PSB-6 in a dose-related manner. Among them, ATZ imparted maximum negative effect, where it reduced the bacterial production of IAA, ACC deaminase and 2, 3-DHBA by 68% (p ≤ 0.001), 75% (p ≤ 0.001), and 83% (p ≤ 0.005), respectively, over control. Additionally, following herbicide exposure, bacterial counts (log10 CFU mL−1) were reduced. Higher concentrations of ATZ and BCR completely reduced the growth patten of strain PSB-6. The current investigation provides an insight into a mechanistic approach of chemical herbicide-induced hazard toward a beneficial soil isolate. Careful monitoring is therefore necessary before agricultural application of pesticides.KEYWORDS: Pesticides stressRhodococcus erythropolisEnzymatic profileBacterial activityCellular viabilityActive biomolecules AcknowledgementsThe authors extend their appreciation to the Researchers Supporting Project number (RSP2023R367), King Saud University, Saudi Arabia.Disclosure statementNo potential conflict of interest was reported by the author(s).Author contributionsConceptualisation, M.S. and M.S.K,; methodology, M.S.; software, M.S., A.S.; validation, M.S., M.S.K and A.S; formal analysis, M.S and M.S.K., and A.M.E.; investigation, M.S. and M.S.K; resources, M.S., M.S.K., A.S and A.M.E.; data curation, M.S.; writing – original draft preparation, M.S., and M.S.K; writing – review and editing, M.S., J.P., and M.S.K.; visualisation, M.S.K., and J.P.; supervision, M.S.K.; project administration, M.S. and M.S.K; funding acquisition, A.S., A.M.E., and L.S.W. All authors have read and agreed to the published version of the manuscript.Additional informationFundingThis research was funded by the Researchers Supporting Project number [RSP2023R367], King Saud University, Saudi Arabia.Notes on contributorsMohammad ShahidMohammad Shahid is a postdoctoral fellow at ICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM), Mau, U.P, India. He obtained his PhD (Agricultural Microbiology) from Aligarh Muslim University, Aligarh, India. He has published a large number of papers including research and review on microbiology, plant-microbe interaction, rhizosphere biology and pesticide toxicology in various reputed journals. He has attended several national and international conferences, seminars, and workshops. He is reviewer of several national and international journals.Mohammad Saghir KhanMohammad Saghir Khan is a full Professor at the Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, India. Dr. Khan received his Ph.D. (Microbiology) degree from Govind Ballabh Pant University of Agriculture & Technology, Pant Nagar, India. He has been teaching Microbiology to post-graduate students for the last 25 years and has research experience of 29 years. In addition to his teaching, Dr. Khan is engaged in guiding students for their doctoral degree in Microbiology and has successfully supervised 12 research scholars for Ph.D. degree in Agricultural Microbiology while five research scholars are working under his supervision. He has published over 200 scientific papers with a total citation of 17484 (google citation; h- index 55; i10 index 240) including, original research articles, review articles and book chapters in various national and international publication media. Some of the notable journals in which Dr. Khan has published his work include International Journal of Nanomedicines, Chemosphere, Ecotoxicology, Environmental Pollution, Environmental Chemistry letters, Scientific Reports, Current Microbiology, Microbiological Research, Crop Protection, Pesticide Biochemistry & Physiology, ACS Omega, Food and Chemical Toxicology, European Journal of Agronomy, Archives of Environmental Contamination & Toxicology etc. Dr. Khan has edited eleven books published by the leading international publishers. Of these books, a total of 176535 book chapters have been downloaded worldwide. Dr. Khan has also completed research projects funded by UGC and ICAR. His major research area includes Ecotoxicology, Nanotoxicology, Bioremediation, Environmental Microbiology, Rhizobiology and Immunology. Dr. Khan has also served in different administrative capacities in the university.Asad SyedAsad Syed is an Associate Professor at Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia. He has been awarded Doctorate from the University of Pune, whereas he has completed his research work from CSIR-National Chemical Laboratory, Pune, India, where he served as Research Associate and Senior Research Fellow in the Biochemical Sciences Division. His research interest is in fabrication of nanomaterials, by utilizing fungi, bacteria, plant extracts, protein, etc. Another area of his expertise is secondary metabolite from endophytic fungi. Now he ventured into the potential application of nanomaterials in environment and healthcare. He is serving as reviewer for international peer journals that include materials science and microbiology journals. He is teaching basic microbiology and laboratory skills to bachelor's students and involved in mentoring master's and PhD students at Botany and Microbiology Department, KSU, Riyadh. He has published his findings in international peer-reviewed materials science and microbiology journals.Abdallah M. ElgorbanAbdallah M. Elgorban is an Associate Professor at Centre of Excellence in Biotechnology Research (CEBR), King Saud University, Riyadh, Saudi Arabia from March 2009 till date, with 24 years of research and academic experience. He has published 320 international research papers in peer reviewed international journal, with high impact factors such as 24.3 (Fungal Diversity, Top%1), and also having good impact on Publon as 28 h-index and 4500 citations for published papers. His research interest falls in the areas as Nanotechnology, Microbiology, Biotechnology and Molecular Biology. He has an experience and keen interest to learn and explore varied techniques from biotechnology and nanotechnology.John PichtelJohn Pichtel PhD, CHMM, is a professor in Environment, Geology, and Natural Resources, Ball State University, Muncie, IN 47306, US. Prof. Pichtel received his Ph. D in Agronomy/ Environmental Science from the Ohio State University, Columbus, OH in 1987. He did his programme in Agronomy/Soil Chemistry from the Ohio State University, Columbus, OH in 1983. He completed his BS degree in Natural Resource Management from Rutgers University, New Brunswick, NJ. 1980. His research embraces management of hazardous materials, remediation of contaminated sites, and environmental chemistry. His research group is studying the behaviour of metallic nanoparticles in soil and aquatic environments. He is particularly interested in assessing shifts in microbial communities and changes in plant physiological processes as a result of interaction with nanoparticles. They have evaluated soil chemical properties and plant response during restoration of brownfields and Superfund sites in the United States as well as highly contaminated environs in the United Kingdom, Ireland, Finland, and Thailand. Other research involves investigation of microbially-catalysed decomposition of PCBs, explosives, propellants, and other xenobiotics.Ling Shing WongLing Shing Wong is graduated from The National University of Malaysia (UKM), Bangi, Selangor, Malaysia in 2010 with Ph.D. in Environmental Science, specialized in the development of biosensor for environmental toxicity assessment. He is currently a professor in INTI International University, Nilai, Negeri Sembilan, Malaysia. His research interests include development of biosensor for environmental application, discovery of new reporter groups for bioindicator, as well as algae and cyanobacteria culture technology. He has authored and co-authored more than 130 scientific articles and extends his service as editorial member in many international journals. Dr. Wong is a senior member of Asia-Pacific Chemical, Biological & Environmental Engineering Society (APCBEES) and the Institute of Research Engineers & Doctors (theIRED), Member of Persatuan Sains Analisis Malaysia and Persatuan Pembangunan Sensor Malaysia. He won several innovation awards including a bronze medal from Malaysia Technology Expo (MTE), Kuala Lumpur, Malaysia in 2006, a gold medal from International Invention, Innovation & Technology Exhibition (ITEX), Kuala Lumpur, Malaysia in 2008, a gold medal from Product Design Competition (PRODEC) in 2013, a silver medal from International Innovation Festival (Innofest) in 2014, finalist of National Outstanding Innovator Award in 2015, a silver medal from Private Higher Education Invention and Innovation Contest (PERINTIS) in 2016, a silver medal from International Invention & Innovation Competition in 2017, two bronze medal from International Invention, Innovation and Design Expo (INNODEX) in 2021, and a silver medal from Malaysia Technology Expo (MTE) in 2021.
除草剂对已鉴定红红红球菌根际分离株细胞生长、微生物学和生理活性的影响
摘要针对目前环境中农药污染严重的现状,选择阿特拉津(ATZ)、丁草胺(BCR)和对乙quizalofp -p-ethyl (QUIZ)对红红红球菌PSB-6的毒性作用进行了评价。MG028649)。测定了ATZ、BCR和QUIZ对红褐霉的MIC值分别为100、200和150 μM。生物标志物酶分析包括LPO、LDH和除草剂诱导的氧化应激(CAT)对菌株PSB-6具有显著(p≤0.005)的毒性。除草剂诱导的形态改变,即边缘异常;扫描电镜观察红胎鼠细胞出现明显的细胞破裂和变形损伤。此外,经除草剂处理和DAPI(4′,6-二氨基-2-苯基吲哚)染色的细胞在CLSM下显示出浓度依赖性的细胞通透性降低。此外,除草剂对PSB-6的生物活性分子表现出剂量相关的毒性。其中,ATZ的负作用最大,与对照相比,ATZ使IAA、ACC脱氨酶和2,3 - dhba的细菌产量分别减少68% (p≤0.001)、75% (p≤0.001)和83% (p≤0.005)。此外,除草剂暴露后,细菌计数(log10 CFU mL−1)减少。较高浓度的ATZ和BCR完全降低了菌株PSB-6的生长模式。目前的研究为化学除草剂对有益土壤分离物的危害提供了一种机制方法。因此,在农业使用农药之前,必须仔细监测。关键词:农药胁迫红嗜红球菌酶谱细菌活性细胞活力活性生物分子感谢沙特阿拉伯沙特国王大学研究人员支持项目(RSP2023R367)披露声明作者未报告潜在的利益冲突。作者贡献概念化,M.S.和m.s.k.;方法,硕士;软件,硕士,硕士;验证、ms、ms和as;形式分析,M.S和M.S.K,以及A.M.E.;调查,M.S.和M.S.K;资源,m.s., m.s.k., a.s.和A.M.E.;数据管理硕士;写作-原始草稿准备,m.s.和M.S.K;写作-审查和编辑,文学硕士,法学硕士和文学硕士;可视化,m.s.k.和J.P.;监督M.S.K.;项目管理,硕士和硕士;所有作者均已阅读并同意稿件的出版版本。本研究由沙特阿拉伯沙特国王大学研究人员支持项目号[RSP2023R367]资助。mohammad Shahid是icar -国家农业重要微生物局(naim)的博士后研究员,该局位于印度北方邦Mau。他在印度阿里格尔穆斯林大学获得农业微生物学博士学位。在国内外知名期刊上发表了大量关于微生物学、植物与微生物相互作用、根际生物学、农药毒理学等方面的研究和综述论文。他参加过几次国内和国际会议、研讨会和讲习班。他是几家国内和国际期刊的审稿人。Mohammad Saghir Khan是印度阿里加尔穆斯林大学农业微生物系的全职教授。他获得了印度Govind Ballabh Pant农业与技术大学的微生物学博士学位。从事研究生微生物学教学25年,科研经验29年。除了他的教学,他还从事指导学生攻读微生物学博士学位,并成功地指导了12名农业微生物学博士学位的研究学者,同时有5名研究学者在他的指导下工作。发表学术论文200余篇,总被引次数17484次;H -指数55;I10索引240),包括在各种国内和国际出版媒体上发表的原创研究文章、评论文章和书籍章节。Khan博士发表的一些著名期刊包括国际纳米医学杂志、化学圈、生态毒理学、环境污染、环境化学快报、科学报告、当代微生物学、微生物研究、作物保护、农药生物化学与生理学、ACS Omega、食品与化学毒理学、欧洲农学杂志、环境污染与毒理学档案等。汗博士编辑了11本由国际领先出版商出版的书籍。在这些书中,全球共有176535本书章节被下载。汗博士亦完成由教资会及ICAR资助的研究项目。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Chemistry and Ecology
Chemistry and Ecology 环境科学-环境科学
CiteScore
4.20
自引率
16.00%
发文量
58
审稿时长
3.7 months
期刊介绍: Chemistry and Ecology publishes original articles, short notes and occasional reviews on the relationship between chemistry and ecological processes. This journal reflects how chemical form and state, as well as other basic properties, are critical in their influence on biological systems and that understanding of the routes and dynamics of the transfer of materials through atmospheric, terrestrial and aquatic systems, and the associated effects, calls for an integrated treatment. Chemistry and Ecology will help promote the ecological assessment of a changing chemical environment and in the development of a better understanding of ecological functions.
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