Investigation on growth modeling and kinetic analysis of lead-resistant bacteria Bacillus infantis isolated from battery industry waste mud

IF 1 Q4 ENGINEERING, ENVIRONMENTAL

Journal of Environmental Engineering and Science Pub Date : 2023-10-05 DOI:10.1680/jenes.23.00035

Moumita Bose, Tapobrata Dey

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Abstract

The present investigation introduces a novel approach on advanced biological remediation for heavy metal lead removal from industrial waste. The ongoing research collaborating theoretical insights with experimentally-derived data to develop strategies to operate bioreactor proficiently on large scale mode. Initially bacteria naturally well equipped with lead resistance has been isolated and identified from native source 16srDNA study along with other microbiological tests have been conducted with the isolated lead resistant bacterial cell and it has been revealed that the isolated lead resistant cell is Bacillus infantis 4352-1T. An extensive studies regarding cell growth of the candidate cell has been carried out in batch mode using well plugged 100 ml conical flasks placed on a rotary shaker under aseptic condition to determine the activity of Bacillus infantis 4352-1T towards its lead removal.Then an attempt has been made to set out to formulate an equation that characterizes the growth kinetics of the lead-resistant Bacillus infantis 4352-1T under lead microenvironment. Through a combination of theoretical analysis and experimental data, it has been observed that Monod’s equation accurately describes the cell growth progress within concentration range of lead (0.05-0.25 kg lead/m 3 ). In this range a theoretical analysis with the help of experimental data is not only confirmed the validity of Monods equation within the concentration range (0.05-0.25 kg lead/m 3 ) but also helped to derive the maximum specific cell growth rate 0.0237 hr −1 and substrate saturation constant 0.018 kg/m 3 . Interestingly, further experiments has been conducted with lead concentrations beyond 0.25 kg lead/m 3 and upto 0.43 kg lead/m 3 show a swift declining in the specific cell growth rate signifying pronounced effect of substrate inhibition clarified through Haldane equation quantitatively.

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电池工业废泥中抗铅细菌婴儿芽孢杆菌的生长模型研究及动力学分析

介绍了一种新型的工业废水重金属铅的高级生物修复方法。正在进行的研究将理论见解与实验数据相结合，以制定在大规模模式下熟练操作生物反应器的策略。最初，从天然来源中分离并鉴定了天然耐铅细菌16srDNA研究以及其他微生物学测试，并对分离的耐铅细菌细胞进行了研究，结果表明分离的耐铅细胞是婴儿芽孢杆菌4352-1T。在无菌条件下，对候选细胞的细胞生长进行了广泛的研究，使用密封良好的100毫升锥形烧瓶放在旋转摇床上，以确定婴儿芽孢杆菌4352-1T对其铅去除的活性。然后试图建立耐铅婴儿芽孢杆菌4352-1T在铅微环境下的生长动力学方程。通过理论分析和实验数据的结合，观察到Monod方程准确地描述了铅浓度范围(0.05-0.25 kg铅/ m3)内细胞的生长过程。在此浓度范围内，理论分析和实验数据不仅证实了Monods方程在浓度范围(0.05-0.25 kg铅/m 3)内的有效性，而且还有助于推导出最大特定细胞生长速率0.0237 hr−1和底物饱和常数0.018 kg/m 3。有趣的是，在铅浓度超过0.25 kg铅/ m3和高达0.43 kg铅/ m3的情况下进行的进一步实验表明，特定细胞生长速率迅速下降，这表明通过霍尔丹方程定量澄清了底物抑制的明显影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Environmental Engineering and Science 环境科学-工程：环境

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

12 months

期刊介绍： Journal of Environmental Engineering and Science is an international, peer-reviewed publication providing a forum for the dissemination of environmental research, encouraging interdisciplinary research collaboration to address environmental problems. It addresses all aspects of environmental engineering and applied environmental science, with the exception of noise, radiation and light.