Comparative Study of Cellulose Tris(3-chloro-5-methylphenylcarbamate) Coated or Covalently Immobilized on Silica for the Separation of Enantiomers in High-Performance Liquid Chromatography

IF 1.2 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2023-11-07 DOI:10.1007/s10337-023-04295-8

Tamar Khatiashvili, Iza Matarashvili, Marina Karchkhadze, Tivadar Farkas, Bezhan Chankvetadze

引用次数: 0

Abstract

In the present study, a comparison was performed between chiral stationary phases (CSPs) prepared by coating or covalent immobilization of cellulose tris(3-chloro-5-methylphenylcarbamate) onto silica. The columns were evaluated in HPLC separation of enantiomers of various acidic, basic, and neutral chiral analytes in acetonitrile, methanol, and mixtures of n-hexane and propan-2-ol as mobile phases. A difference in enantiomer-resolving ability of coated and covalently immobilized CSs was observed.

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涂覆或共价固定在二氧化硅上的三(3-氯-5-甲基苯基氨基甲酸酯)纤维素在高效液相色谱法中分离对映体的比较研究

本研究比较了通过在二氧化硅上涂覆或共价固定三（3-氯-5-甲基苯基氨基甲酸酯）纤维素制备的手性固定相（CSP）。在以乙腈、甲醇以及正己烷和 2-丙醇混合物为流动相的高效液相色谱法分离各种酸性、碱性和中性手性分析物的对映体时，对这些色谱柱进行了评估。结果表明，涂层 CS 与共价固定 CS 的对映体解析能力存在差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.