Acute and Subacute Toxicity of Ruta Montana Extract to Female Rats: Effect on Liver, Kidneys and Ovaries

Mahdeb Nadia, Khadidja Attafi, Souha Bouhouhou, Allouni Rima, Abdelouahab Bouzidi
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Abstract

Ruta montana L. is an annual aromatic plant of the family rutaceae. Quantitative analysis of the methanolic crude extract of Ruta montana L. yielded 8.43%, whereas the qualitative analysis revealed the presence of alkaloid or coumarin. The Litchfield and Wilcoxon method calculated the LD50 of the crude methanolic extract of Ruta montana L. in Wistar albino female rats at 393.18 mg/kg. This allows the plant to be classified as moderately toxic. The subacute toxicity study of the methanolic crude extract of Ruta montana L. in female Wistar albino rats treated with 100 mg/kg (≈ 1/4 LD50) and intraperitoneally showed a significant increase in body weight of the rats treated at the 4th week. Animals treated and sacrificed after 30 days showed a disturbance of the relative mass of the organs. Biochemical parameters of hepatic function assessment showed a significant increase in PAL with elevation of AST and ALT, whereas those of renal function revealed a significant decrease in creatinine with an increase in urea. Hematologic parameters recorded a decrease in RBC, HGB and HCT. The histological sections of the treated rats reveal the existence of blood congestion in the central veins and liver tissues, foci of necrosis and steatosis in the liver, blood congestion and some glomerular atrophy in the kidneys, as well as blood congestions and developed follicles without oocytes in the ovaries.
芦花提取物对雌性大鼠的急性和亚急性毒性:对肝脏、肾脏和卵巢的影响
芦花是一种一年生芳香植物,属芦花科。经定量分析,芦花粗提物的甲醇含量为8.43%,定性分析发现芦花粗提物中含有生物碱或香豆素。Litchfield和Wilcoxon法计算出大黄草粗甲醇提取物在Wistar白化雌性大鼠体内的LD50为393.18 mg/kg。这使得这种植物被归类为中等毒性。对雌性Wistar白化大鼠给予100 mg/kg(≈1/4 LD50)乙醇粗提物并腹腔注射的亚急性毒性研究显示,第4周时大鼠体重显著增加。治疗后30天处死的动物表现出器官相对质量的紊乱。肝功能生化指标显示PAL随AST和ALT升高而显著升高,肾功能生化指标显示肌酐随尿素升高而显著降低。血液学指标显示RBC、HGB和HCT下降。给药大鼠的组织学切片显示中央静脉和肝脏组织充血,肝脏坏死和脂肪变性灶,肾脏充血和部分肾小球萎缩,卵巢充血和卵泡发育,无卵母细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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