The effect of levofloxacin ointment against imiquimod induced–psoriasis in mice model

Abstract

The goal of this study was to prepare levofloxacin as a topical ointment for evaluating its anti-psoriatic activity in imiquimod-induced psoriasis in mice. Sixty mice 24 – 30 grams were randomly divided into six groups of 10 mice each. The first group was a control group, in which the baseline ointment was application to the shaved back. The second group received imiquimod (5%) cream for 6 days on back in the same way as the remaining four groups to induce psoriasis of skin inflammation. The 3rd, 4th, 5th and 6th groups were treated with application of imiquimod daily clobetasol, levofloxacin 10, 20 and 40% ointment. At the end of the experiment, blood was obtained to prepare serum for measuring inflammatory biomarkers, while skin samples were used to study histopathological changes. Levofloxacin ointment dramatically reduced the scores of erythematous, scaling, and epidermal thickenings, as well as the inflammatory cytokines TNF-α, IL-8, IL-17, and IL-37. Levofloxacin ointment has strong anti-psoriatic and anti-inflammatory activities.