Comparison of fibroblast-growth factor-23 level between males and female’s broiler using HPLC technique

Abstract

Animal public health is crucial concern when considering the role of fibroblast growth factor-23 (FGF-23) in regulating phosphate metabolism. FGF-23 has been associated with to various diseases in animal, such as chronic kidney and cardiovascular disease, making it a crucial factor to consider for maintaining the well-being of animals and ensuring public health. This study aimed to measure the levels of FGF-23 protein in broiler chickens and compare them to a standard level of 375 pg/ml. Blood samples were collected from 10 female and 10 male broiler chickens, and FGF-23 protein levels were analyzed using high-performance liquid chromatography (HPLC). The results showed significant gender differences in FGF-23 protein levels, with males having a higher level of FGF-23 protein than females. Females had a mean level of 256.12 pg/ml (SD = 86.02), while males had a significantly higher mean level of 1508.71 pg/mL (SD = 1113.85). However, the study is limited by the small sample size and the use of a single population. Further research with larger and more diverse samples is needed to confirm the generalizability of these findings. Conclusion, this study provides important insights into the role of gender in FGF-23 protein metabolism in broiler chickens, and may have implications for the diagnosis and treatment of diseases associated with FGF-23 protein dysregulation in poultry.