Micropropagation of Trichopilia suavis Lindl. & Paxton

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.1590/2447-536x.v29i3.2653

Muthab Hussien, Olga Molkanova, Irina Vjacheslavovna Mitrofanova

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引用次数: 0

Abstract

Abstract Trichopilia suavis is one of the important representatives of the genus Trichopilia, which can be used as a potted plant for the interior design. also, its beautiful inflorescences are added unusual element to bouquet compositions and actively used in the production of perfumes. This study was carried out in order to develop and improve micropropagation method for mass clonal production of T. suavis through protocorm culture. Half-strength Murashige and Skoog culture medium (½) has been modified by adding different organic components and growth regulators. The protocorms obtained from seeds germinated by asymbiotic method were used as explants. The results showed that the optimal treatment for formation of new protocorms (7.50 ± 0.7 units per protocorm) was obtained by culture on ½ MS medium with 0.5 mg L-1 of 6-Benzylaminopurine (6-BAP) and 100 mL L-1 of coconut water. At the stage of rooting, the highest number and length of roots (7.0 ± 0.45 units per plantlet, 2.64 ± 0.18 cm) were obtained through subsequent cultivation of plantlets on ½ MS medium with 0.5 mg L-1 indolyl-3-butyric acid (IBA), 1.0 g L-1 charcoal with the addition of 50 g L-1 of banana puree. The obtained seedlings were successfully adapted in a substrate consisting of bark, perlite and peat in a ratio of 1:1:1.

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藜毛霉的微繁研究。,帕克斯顿

摘要:毛头木是毛头木属植物的重要代表之一，可作为室内设计的盆栽植物。此外，它美丽的花序被添加到花束成分中，并积极用于香水的生产。本研究旨在通过原球茎培养，建立和完善棉叶甜菊大量无性系的繁殖方法。通过添加不同的有机成分和生长调节剂对半强度Murashige和Skoog培养基(½)进行了改性。以非共生萌发法获得的原球茎为外植体。结果表明，在0.5 mg L-1 6-苯氨基opurine (6-BAP)和100 mL L-1椰子水的½MS培养基上培养新原球茎的最佳处理为7.50±0.7个单位/原球茎。在生根阶段，在0.5 mg L-1吲哚基-3-丁酸(IBA)、1.0 g L-1木炭和50 g L-1香蕉泥的½MS培养基上进行后续培养，获得了最高的根数和根长(7.0±0.45个/株，2.64±0.18 cm)。将获得的幼苗成功地适应于由树皮，珍珠岩和泥炭组成的基质中，其比例为1:1:1。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.