Effect of 1-butyl-3-methylimidazolium tetrafluoroborate on photosynthesis in Arabidopsis thaliana

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-09-15 DOI:10.1080/11263504.2023.2258882

Lu Gao, Charles Obinwanne Okoye, Yanfang Wu, Guizhu Wu, Jianxiong Jiang, Daolin Du, Yonglai Xue

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引用次数: 0

Abstract

ABSTRACTIonic liquids (ILs) are a promising alternative to the traditional organic solvents, but their toxic effects cause significant adverse environmental effects and their toxicity assessment have been suggested. The effect of the ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim][BF4]) on photosynthesis was studied in Arabidopsis thaliana. Apparent effects on chlorosis and inhibition of photosynthetic capacity were observed. As [Bmim][BF4] concentrations increased, a time-dose effect was also observed. The maximum quantum yield (Fv/Fm), and the actual quantum yield of photosystem II (ΦPSII), both decreased; at the same time, the expression of psbA, a key gene of the photosystem II electron transport, was reduced by [Bmim][BF4] treatment. The synthesis of the large and small subunits of Rubisco were not affected by treatment. On the other hand, Rubisco activase (RCA) was significantly suppressed at the transcriptional level. Our results suggest that photochemistry of PSII as well as Rubisco activation are involved in ILs resistance.Key words: Arabidopsis thaliana; [Bmim][BF4]Chlorophyll fluorescencegene transcript levelDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also. Author contributionsLu Gao, Charles Obinwanne Okoye, and Guizhu Wu designed the study, performed the experiments, and wrote the article. Yanfang Wu and Jianxiong Jiang supervised the study. Daolin Du and Yonglai Xue conceived the study, reviewed and edited the manuscript. All authors read and approved the manuscript.AcknowledgmentsThis work was supported by the Key Research and Development Program of Zhenjiang City (SH20222011), and the Startup Support from Jiangsu University (13JDB017).

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1-丁基-3-甲基咪唑四氟硼酸盐对拟南芥光合作用的影响

摘要离子液体是传统有机溶剂的一种很有前途的替代品，但其毒性对环境有显著的不良影响，其毒性评价已被提出。研究了离子液体1-丁基-3-甲基咪唑四氟硼酸盐([Bmim][BF4])对拟南芥光合作用的影响。对黄化和抑制光合能力有明显影响。随着[Bmim][BF4]浓度的增加，还观察到时间剂量效应。光系统II的最大量子产率(Fv/Fm)和实际量子产率(ΦPSII)均下降;同时，[Bmim][BF4]处理降低了光系统II电子传递的关键基因psbA的表达。Rubisco大小亚基的合成不受处理的影响。另一方面，Rubisco激活酶(RCA)在转录水平上被显著抑制。我们的研究结果表明PSII的光化学反应和Rubisco的活化参与了对il的抗性。关键词:拟南芥;[Bmim][BF4]叶绿素荧光基因转录水平免责声明作为对作者和研究人员的服务，我们提供此版本的已接受稿件(AM)。在最终出版版本记录(VoR)之前，将对该手稿进行编辑、排版和审查。在制作和印前，可能会发现可能影响内容的错误，所有适用于期刊的法律免责声明也与这些版本有关。lu Gao, Charles Obinwanne Okoye和Guizhu Wu设计了这项研究，进行了实验，并撰写了这篇文章。吴艳芳和姜建雄监督了这项研究。杜道林、薛永来负责研究构思、审稿和编辑工作。所有作者都阅读并认可了稿件。本研究得到镇江市重点研发计划(SH20222011)和江苏大学创业基金(13JDB017)的支持。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.