Sahra Genc, Sally Thompson, Owen Hill, Leif Gislason, Dakota Rodriguez, Farjana Showme, Alex Motler, Sarah M. Schreiner, Adrian Gestos, Virginia L. Ferguson, Jeff Jessing
{"title":"Morphological and mechanical characterization of a novel porous silicon membrane used in a lung-on-a-chip system","authors":"Sahra Genc, Sally Thompson, Owen Hill, Leif Gislason, Dakota Rodriguez, Farjana Showme, Alex Motler, Sarah M. Schreiner, Adrian Gestos, Virginia L. Ferguson, Jeff Jessing","doi":"10.1557/s43580-023-00667-2","DOIUrl":null,"url":null,"abstract":"Abstract In the last decade, organ-on-a-chip technology has been researched as an alternative to animal and cell culture models (Buhidma et al. in NPJ Parkinson’s Dis, 2020; Pearce et al. in Eur Cells Mater 13:1–10, 2007; Huh et al. in Nat Protoc 8:2135–2157, 2013). While extensive research has focused on the biological functions of these chips, there has been limited exploration of functional materials that can accurately replicate the biological environment. Our group concentrated on a lung-on-a-chip featuring a newly fabricated porous silicon bio-membrane. This bio-membrane mimics the interstitial space found between epithelial and endothelial cells in vivo, with a thickness of approximately 1 μm (Ingber in Cell 164:1105–1109, 2016). This study aims to establish a fabrication method for producing a thin, uniform porous silicon membrane with a predictable reduced modulus . We conducted mechanical and morphological characterization using scanning electron microscopy and nanoindentation. A small, parametric study was conducted to determine the reduced modulus of the porous silicon and how it may relate to the morphological features of the membrane. We compare our results to other works. Graphical Abstract","PeriodicalId":19015,"journal":{"name":"MRS Advances","volume":"35 7","pages":"0"},"PeriodicalIF":0.8000,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"MRS Advances","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1557/s43580-023-00667-2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract In the last decade, organ-on-a-chip technology has been researched as an alternative to animal and cell culture models (Buhidma et al. in NPJ Parkinson’s Dis, 2020; Pearce et al. in Eur Cells Mater 13:1–10, 2007; Huh et al. in Nat Protoc 8:2135–2157, 2013). While extensive research has focused on the biological functions of these chips, there has been limited exploration of functional materials that can accurately replicate the biological environment. Our group concentrated on a lung-on-a-chip featuring a newly fabricated porous silicon bio-membrane. This bio-membrane mimics the interstitial space found between epithelial and endothelial cells in vivo, with a thickness of approximately 1 μm (Ingber in Cell 164:1105–1109, 2016). This study aims to establish a fabrication method for producing a thin, uniform porous silicon membrane with a predictable reduced modulus . We conducted mechanical and morphological characterization using scanning electron microscopy and nanoindentation. A small, parametric study was conducted to determine the reduced modulus of the porous silicon and how it may relate to the morphological features of the membrane. We compare our results to other works. Graphical Abstract
在过去的十年中,器官芯片技术被研究作为动物和细胞培养模型的替代方法(Buhidma et al. In NPJ Parkinson 's Dis, 2020;Pearce et al. journal of chengdu electro - mechaical, 2007;Huh et al. in Nat Protoc 8:2135-2157, 2013)。虽然广泛的研究集中在这些芯片的生物功能上,但对能够准确复制生物环境的功能材料的探索有限。我们的团队专注于一种肺芯片,其特点是一种新制造的多孔硅生物膜。这种生物膜模拟了体内上皮细胞和内皮细胞之间的间隙,厚度约为1 μm (Ingber in Cell 164:1105-1109, 2016)。本研究旨在建立一种制造方法,用于生产具有可预测的降低模量的薄而均匀的多孔硅膜。我们使用扫描电子显微镜和纳米压痕进行了机械和形态表征。一个小的,参数化的研究进行了确定减少的多孔硅的模量,以及它如何可能涉及到膜的形态特征。我们将我们的结果与其他作品进行比较。图形抽象