Simultaneous Quantitative Determination of Eight Fluoroquinolones by High-Performance Liquid Chromatography with UV Detection using a C18 Monolithic Column

IF 0.7 Q4 CHEMISTRY, ANALYTICAL

Methods and Objects of Chemical Analysis Pub Date : 2023-01-01 DOI:10.17721/moca.2023.101-108

Emna Toujani, Wafa Belgaied, Sana Toujani

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Abstract

In the present work, an accurate and reliable reversed-phase liquid chromatographic method (RP-HPLC) for the simultaneous quantitative determination of eight fluoroquinolones (enoxacin, norfloxacin, ciprofloxacin, levofloxacin, moxifloxacin, enrofloxacin, sparfloxacin, and marbofloxacin) in bulk form and in pharmaceutical products is described. The optimum separation of the selected pharmaceutical compounds was achieved on a Chromolith® RP-18 endcapped column with a mobile phase consisting of acetonitrile and phosphate buffer (pH 5.5; 15 mM) pumped at 2 mL min-1. UV detection of all compounds was accomplished at 280 nm. The procedure was validated according to the International Conference on Harmonization (ICH) Q2 (R1) guideline. Good linear relationships were obtained (r ≥ 0.996) for all the analytes over their respective concentration ranges. Intra- and inter-day precision were less than 2 %. Recoveries were held in the range of 98 to 102 %. The developed method was successfully applied to the analysis of pharmaceutical products.

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C18整体柱高效液相色谱-紫外检测同时定量测定8种氟喹诺酮类药物

本文建立了一种准确可靠的反相液相色谱法(RP-HPLC)，用于同时定量测定原料药和制剂中8种氟喹诺酮类药物(依诺沙星、诺氟沙星、环丙沙星、左氧氟沙星、莫西沙星、恩诺沙星、斯帕沙星和马布沙星)的含量。在所选药物化合物的最佳分离是在Chromolith®RP-18端盖柱上实现的，流动相为乙腈和磷酸盐缓冲液(pH 5.5;15mm)泵在2ml min-1。所有化合物的紫外检测均在280 nm完成。该程序根据国际协调会议(ICH) Q2 (R1)指南进行验证。各分析物在各自浓度范围内均具有良好的线性关系(r≥0.996)。日内和日内精度均小于2%。加样回收率在98% ~ 102%之间。该方法已成功地应用于药品的分析。

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来源期刊

Methods and Objects of Chemical Analysis CHEMISTRY, ANALYTICAL-

CiteScore

1.00

自引率

14.30%

发文量

期刊介绍： The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.