Development of the Spectrophotometric Method for the Determination of Metoprolol in Tablets by using Bromothymol Blue

IF 0.7 Q4 CHEMISTRY, ANALYTICAL

Methods and Objects of Chemical Analysis Pub Date : 2023-01-01 DOI:10.17721/moca.2023.63-69

Mariana Horyn, Marjan Piponski, Oleksandra Melnyk, Iryna Ivanchuk, Oleksandr Susla, Liliia Grytsyshyn, Dmytro Korobko, Liliya Logoyda

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Abstract

A simple, eco-friendly, rapid, and economic spectrophotometric method for the determination of metoprolol tartrate in tablets has been developed. The procedure for metoprolol determination is based on a reaction with bromothymol blue (BTB) in acetonitrile medium. The maximum formation of the colored product was observed in acetonitrile at the absorption maximum at a wavelength of 402 nm. The stoichiometry of molecular complexes was determined by Job's method and it was found to be 1 to 1. The optimal concentration of BTB was 1.6·10-4 M. The linear relationship between absorbance at λmax and concentration of analyte ranging 16.00-24.00 μg mL-1 was found. Regression analysis of Beer’s law plot at 402 nm yielded the regression equation, y = 0.0199x + 0.0721. High values of correlations coefficient R2 = 0.9990 and small values of intercept validated the linearity of calibration curve and obedience to Beer’s law. The calculated LOD and LOQ values were 1.90 and 5.78 μg mL-1, respectively. The proposed green spectrophotometric method is simple, rapid, and suitable for routine pharmaceutical analysis.

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溴百里酚蓝分光光度法测定片剂中美托洛尔的含量

建立了一种简单、环保、快速、经济的分光光度法测定酒石酸美托洛尔片剂中酒石酸美托洛尔的含量。美托洛尔的测定方法是在乙腈介质中与溴百里酚蓝(BTB)反应。在402 nm的吸收峰处，乙腈中有色产物的形成量最大。分子络合物的化学计量学用约伯法测定，结果为1比1。BTB的最佳浓度为1.6·10-4 m， λmax吸光度与分析物浓度在16.00-24.00 μg mL-1范围内呈线性关系。对402 nm处的比尔定律图进行回归分析，得到回归方程y = 0.0199x + 0.0721。相关系数R2 = 0.9990高，截距值小，说明标定曲线线性，符合比尔定律。定量限和定量限分别为1.90和5.78 μ mL-1。该方法简便、快速，适用于常规药物分析。

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来源期刊

Methods and Objects of Chemical Analysis CHEMISTRY, ANALYTICAL-

CiteScore

1.00

自引率

14.30%

发文量

期刊介绍： The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.