Advances in analysis of microplastics in drinking water treatment plants. Fluorescence techniques using iDye Pink

Pablo Alonso-Vázquez, María-José Lujan-Facundo, Beatriz-Elena Cuartas-Uribe, Amparo Bes-Piá, José-Luis Alonso-Molina, José-Antonio Mendoza-Roca
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Abstract

Recently, the increasing amount of plastic waste has raised concerns about microplastics in aquatic environments. In this study, microplastics between 0.1 and 5 mm in samples from different points of three Drinking Water Treatment Plants (DWTP) were separated, quantified, and identified. Staining methods were used in combination with microscopic and spectroscopic techniques. On the one hand, the Rose Bengal dye was used to discriminate between natural and synthetic particles. On the other hand, Nile Red and iDye Pink reagents have been evaluated for staining microplastics, providing them with fluorescence. The nature of the particles is determined by comparison with a series of patterns by means of an epifluorescence microscope. In addition, "Micro Fourier Transform Spectrophotometer " and "Raman Spectroscopy" were used to identify the nature of the particles more accurately. Concerning the results, polyester was the most common material by fluorescence identification, and it was confirmed with Raman spectroscopy. Since most of the particles observed were microfibres, Raman proved to be a better identification technique than μ-FTIR, which could only identify large fragments. In addition, the global elimination of microplastics (MPs) resulted notable in the three DWTPs, being 81.47% for A, 88.98% for B, and 82.27% for C, thus guaranteeing a higher quality of drinking water.

Abstract Image

饮用水处理厂微塑料分析研究进展。使用iDye Pink的荧光技术
最近,越来越多的塑料垃圾引起了人们对水生环境中微塑料的关注。在本研究中,对来自三个饮用水处理厂(DWTP)不同地点的样品中0.1至5毫米的微塑料进行了分离、定量和鉴定。染色方法与显微和光谱技术相结合。一方面,孟加拉玫瑰染料被用来区分天然和合成颗粒。另一方面,Nile Red和iDye Pink试剂已被评估用于染色微塑料,为其提供荧光。粒子的性质是通过与荧光显微镜下的一系列图案进行比较来确定的。此外,利用“微傅立叶变换分光光度计”和“拉曼光谱”更准确地鉴定了颗粒的性质。结果表明,聚酯是荧光鉴定中最常见的材料,拉曼光谱也证实了这一点。由于观察到的颗粒大部分是微纤维,拉曼被证明是比μ-FTIR更好的识别技术,μ-FTIR只能识别大碎片。此外,微塑料(MPs)的全球消除在三个dwtp中显著,A为81.47%,B为88.98%,C为82.27%,从而保证了更高的饮用水质量。
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