Cat presumptive zygotes assessment in relation to their development

IF 1.9 4区农林科学 Q1 Veterinary

Annals of Animal Science Pub Date : 2023-10-23 DOI:10.2478/aoas-2023-0096

Barbara Kij-Mitka, Julia Gabryś, Joanna Kochan, Agnieszka Nowak, Tomasz Szmatoła, Sylwia Prochowska, Wojciech Niżański, Monika Bugno-Poniewierska

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Abstract

Abstract The evaluation of oocytes and zygotes, based on their size, shape and morphology, is a valuable tool for predicting subsequent embryo development. While this assessment is non-invasive and made possible with time-lapse monitoring systems, not all the assessment criteria used for zygotes with pale cytoplasm can be used for domestic cat zygotes, which have dark cytoplasm. In this study, feline presumptive zygotes were evaluated for shape, size, and morphology. Measurements were also made of the diameter of the entire zygote, its cytoplasm, and the zona pellucida. Differences in the dataset were assessed using the generalized linear model (GLM) procedure. While there was no relationship between a combination of the tested parameters with the potential for cleavage, blastocyst development, and hatching, the parameters of the shape and size of the entire oocyte, and of the zona pellucida, were related to the development potential. The results presented in this study indicate that the assessment procedure for human zygotes have to be adjust to be used in the cat model, however the relationship between measurements of the diameter of presumptive feline zygotes and the thickness of zona pellucida with their developmental potential deserve further investigation to optimize assessment of cat presumptive zygotes.

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与受精卵发育有关的推测性评估

根据卵母细胞和受精卵的大小、形状和形态对其进行评价，是预测胚胎后续发育的重要工具。虽然这种评估是非侵入性的，并且可以通过延时监测系统实现，但并非所有用于具有浅色细胞质的受精卵的评估标准都可用于具有深色细胞质的家猫受精卵。在这项研究中，猫推定受精卵的形状，大小和形态进行了评估。还测量了整个受精卵的直径、细胞质和透明带。使用广义线性模型(GLM)程序评估数据集的差异。虽然所测参数的组合与卵裂、囊胚发育和孵化的潜力没有关系，但整个卵母细胞的形状和大小以及透明带的参数与发育潜力有关。本研究结果表明，人类受精卵的评估程序必须进行调整才能用于猫模型，但猫受精卵直径和透明带厚度的测量值与其发育潜力之间的关系值得进一步研究，以优化猫受精卵的评估。

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来源期刊

Annals of Animal Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

3.80

自引率

5.30%

发文量

138

期刊介绍： Annals of Animal Science accepts original papers and reviews from the different topics of animal science: genetic and farm animal breeding, the biology, physiology and reproduction of animals, animal nutrition and feedstuffs, environment, hygiene and animal production technology, quality of animal origin products, economics and the organization of animal production.