Prevalence of antimicrobial resistance genes among Escherichia coli isolated from poultry

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Indian Journal of Animal Sciences Pub Date : 2023-10-06 DOI:10.56093/ijans.v93i10.111002

None ARPIT, M CHANDRA, G KAUR, D NARANG, A K ARORA

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引用次数: 0

Abstract

Antimicrobial resistance has become a global threat. In the poultry industry, antibiotic usage has been widespread and been used for multiple purposes, viz. growth promoters, therapeutic agent and prophylaxis. This usage has probably led to accumulation of antimicrobial resistant genes. A study on presence of antibiotic resistant genes in poultry farms of Ferozepur and Ludhiana, Punjab were undertaken. A total of 50 faecal samples were collected from eight farms. The samples were processed for isolation of E. coli by using selective media, were identified using various biochemical tests and confirmed with the help of PCR. A total of 35 E. coli isolates were obtained and all were subjected to antibiotic sensitivity test against 10 antibiotics. Also, these isolates were subjected to amplification of antibiotic resistance genes, viz. blaTEM, blaSHV, DHAM, MOXM, sul1, dhfrV, aadA, tetA and tetB using published primers. The isolates revealed resistance to penicillin (100%), ampicillin/sulbactum (100%), erythromycin (94.28%), streptomycin (91.4%), tetracycline (60%), chloramphenicol (60%), trimethoprim (51.4%), co-trimoxazole (48.57%), gentamicin (8.5%) and colistin (8.5%). Seven isolates were found to be positive for blaTEM, nine for sulI, four for dhfrV, 11 for aadA and cmlA, respectively, while none of the isolate showed the blaSHV, DHAM, MOXM, tetA and tetB. The present study revealed that the multiple AMR genes may be prevalent among E. coli isolates of poultry origin which needs urgent attention.

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家禽分离的大肠杆菌耐药基因的流行情况

抗菌素耐药性已成为全球性威胁。在家禽业，抗生素的使用已经广泛，并被用于多种目的，即生长促进剂，治疗剂和预防。这种用法可能导致抗微生物基因的积累。对旁遮普省费罗采普尔和卢迪亚纳家禽养殖场的抗生素耐药基因进行了研究。在8个农场共收集了50份粪便样本。对样品进行选择性培养基分离大肠杆菌处理，使用各种生化试验进行鉴定，并借助PCR进行确认。共分离得到35株大肠杆菌，并对10种抗生素进行了药敏试验。此外，利用已发表的引物扩增这些分离株的抗生素耐药基因，即blaTEM、blaSHV、DHAM、MOXM、sul1、dhfrV、aadA、tetA和tetB。对青霉素(100%)、氨苄西林/磺胺(100%)、红霉素(94.28%)、链霉素(91.4%)、四环素(60%)、氯霉素(60%)、甲氧苄啶(51.4%)、复方新诺明(48.57%)、庆大霉素(8.5%)、粘菌素(8.5%)耐药。7株blaSHV、9株sulI、4株dhfrV、11株aadA和cmlA均呈阳性，而blaSHV、DHAM、MOXM、tetA和tetB均未检出。目前的研究表明，多种AMR基因可能在禽源性大肠杆菌分离株中普遍存在，需要引起高度重视。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Animal Sciences 农林科学-奶制品与动物科学

CiteScore

0.60

自引率

25.00%

发文量

220

审稿时长

8 months

期刊介绍： Articles published in The Indian Journal of Animal Sciences encompass a broad range of research topics in animal health and production related to cattle, buffalo, sheep, goat, camel, equines, pig, rabbit, yak, mithun, poultry and fisheries. Studies involving wildlife species and laboratory animal species that address fundamental questions about their biology will also be considered for publication. All manuscripts must present some new development and must be original, timely, significant and scientifically excellent. Papers will be rejected if standards of care of, or procedures performed on animals are not up to those expected of humane veterinary scientists. At a minimum, standards must meet the International Guiding Principles for Biomedical Research involving Animals, as issued by the Council for International Organizations of Medical Sciences. (C.I.O.M.S., c/o WHO, CH 1211 Geneva 27, Switzerland). Articles reporting new animal disease must follow GOI directive as given in detail in Guidelines to Authors.