Establishment and evaluation of a multiplex PCR amplification-based sequencing method for respiratory virus type A

IF 2.1 4区医学 Q3 VIROLOGY

Future Virology Pub Date : 2023-09-01 DOI:10.2217/fvl-2023-0083

Junhong Luo, Yao Huang, Yan Guo, Zixinrong Meng, Kangchen Zhao, Xiaojuan Zhu, Le Zhou, Tao Wu, Qiao Qiao, Yiyue Ge, Qin Xu, Lunbiao Cui

引用次数: 0

Abstract

Aim: This study aimed to establish a simple whole-genome sequencing method for respiratory virus type A (RSVA) used in genomic epidemiological studies. Methods: Multiple primers were designed to amplify overlapping amplicons specific to RSVA. The amplicons were sequenced using Illumina and Nanopore sequencing platforms and the sequencing performances of the two platforms were also compared. Results: The study shows that the developed method could recover almost full genomes of RSVA when Ct values were less than 33 for clinical samples. Conclusion: In this study, a whole-genome sequencing method was developed for RSVA based on multiplex PCR.

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基于多重PCR扩增的呼吸道病毒a型测序方法的建立与评价

目的:建立一种简易的呼吸道a型病毒(RSVA)全基因组测序方法，用于基因组流行病学研究。方法:设计多个引物扩增RSVA特异性重叠扩增子。利用Illumina和Nanopore测序平台对扩增子进行测序，并比较两种平台的测序性能。结果:研究表明，当临床样本的Ct值小于33时，所建立的方法几乎可以恢复RSVA的全基因组。结论:本研究建立了基于多重PCR的RSVA全基因组测序方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future Virology 医学-病毒学

CiteScore

4.00

自引率

3.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.