Genetic diversity of the Rhododendron subsection Ledum species in Sakhalin Island: data from chloroplast DNA markers variability

Q3 Environmental Science

Botanica Pacifica Pub Date : 2023-01-01 DOI:10.17581/bp.2023.12209

Diana R. Iunusova, Maria A. Polezhaeva, Elena A. Marchuk

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Abstract

A high genetic variability was revealed in the populations of the Rhododendron subsection Ledum species complex in Sakhalin. In total, 94 individuals from natural populations of the four sympatric species of Labrador tea (R. tomentosum, R. subarcticum, R. tolmachevii and R. hypoleucum) were analyzed to evaluate the genetic variation in the territory of the Sakhalin Island and in two mainland populations. The haplotype diversity of five chloroplast intergenic spacers (trnH–psbA, petB–petD, 3’trnV (UAC)–ndhC, K2R–K707, atpB–rbcL) was quite high. There were 16 haplotypes (h1–h16) identified; h1, h2 and h4 are the most common, the rest were rare and no species-specific ones were found. Analysis of Molecular Variance (AMOVA) indicate that about 37 % of the genetic diversity is due to among population differences (FST = 0.37, p < 0.001). Molecular phylogenetic analysis based on chloroplast DNA data did not reveal a clear species structure, but revealed a trend towards the isolation of two genetic groups: including a group of samples from the southern and the middle parts of Sakhalin Island.

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库页岛杜鹃花亚段Ledum种的遗传多样性:叶绿体DNA标记变异的数据

库页岛杜鹃花子种群具有较高的遗传变异性。利用拉布拉多茶4个同域种(毛毛茶、亚北极茶、托马切夫茶和亚北极茶)自然居群的94个个体，分析了库页岛地区和2个大陆居群的遗传变异。5个叶绿体基因间间隔物trnH-psbA、petB-petD、3'trnV (UAC) -ndhC、K2R-K707、atpB-rbcL的单倍型多样性较高。共鉴定出16个单倍型(h1 ~ h16);H1, h2和h4是最常见的，其余的是罕见的，没有发现物种特异性。分子方差分析(AMOVA)表明，约37%的遗传多样性是由群体间差异造成的(FST = 0.37, p <0.001)。基于叶绿体DNA数据的分子系统发育分析没有显示出明确的物种结构，但显示出两个遗传群体的分离趋势:包括来自萨哈林岛南部和中部的一组样本。

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来源期刊

Botanica Pacifica Environmental Science-Ecology

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Botanica Pacifica (BP) publishes peer-reviewed, significant research of interest to a wide audience of plant scientists in all areas of plant biology (structure, function, development, diversity, genetics, evolution, systematics), all levels of organization (molecular to ecosystem), and all plant groups and allied organisms (cyanobacteria, fungi, and lichens). BP requires authors to frame their research questions and discuss their results in terms of contemporary problems of plant biology. While the geographic focus of the journal is the Pacific region, research submissions that demonstrate clear linkages with other regions are welcome. BP aims to foster the exchange of research ideas between countries with diverse cultures and languages.