The effect of abiotic factors on in vitro seed germination in Oxytropis chankaensis Jurtz., a rare endemic species of the Russian Far East

Q3 Environmental Science

Botanica Pacifica Pub Date : 2023-01-01 DOI:10.17581/bp.2023.12206

Kseniya S. Berdasova, Anastasiya S. Pianova, Liubov A. Kameneva

引用次数: 0

Abstract

The paper presents the results of different ways of overcoming dormancy of seeds of Oxytropis chankaensis Jurtz., a rare endemic species of the Far East characterized by small isolated populations. The peculiarities of seed germination under the influence of various abiogenic factors were studied: concentrated sulfuric acid, stratification, seed hilum puncture and abrupt temperature difference. A protocol for introducing the species studied to culture in vitro in order to preserve this endemic has been developed. The assessment of seedling viability after exposure to each of the factors was carried out. It was shown that the most effective and fastest way of scarification is the use of concentrated sulfuric acid. Stimulation of seed germination by this factor leads to 100 % seed germination and obtaining more than 90 % of viable plants capable of microclonal multiplication.

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非生物因素对长春棘豆离体种子萌发的影响。俄罗斯远东地区的一种罕见的特有物种

本文介绍了长春棘豆种子克服休眠的不同方法的结果。远东地区一种罕见的特有物种，以小而孤立的种群为特征。研究了浓硫酸、分层、种门穿刺和突变温差等非生物因素对种子萌发的影响。已经制定了将所研究的物种引入体外培养以保存这种地方病的方案。对各因子作用后的幼苗活力进行了评价。结果表明，用浓硫酸进行刻蚀是最有效、最快速的方法。用该因子刺激种子萌发，可使种子萌发率达到100%，获得90%以上具有微克隆繁殖能力的活株。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Botanica Pacifica Environmental Science-Ecology

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Botanica Pacifica (BP) publishes peer-reviewed, significant research of interest to a wide audience of plant scientists in all areas of plant biology (structure, function, development, diversity, genetics, evolution, systematics), all levels of organization (molecular to ecosystem), and all plant groups and allied organisms (cyanobacteria, fungi, and lichens). BP requires authors to frame their research questions and discuss their results in terms of contemporary problems of plant biology. While the geographic focus of the journal is the Pacific region, research submissions that demonstrate clear linkages with other regions are welcome. BP aims to foster the exchange of research ideas between countries with diverse cultures and languages.