Therapeutic Effects of Co-administration of Silver Nanoparticles and Vitamin C on Vaginal Infection Caused by Group B Streptococcus

IF 1.4 Q4 PHARMACOLOGY & PHARMACY
Zhale Mansoori, Khosrow Chehri, Ahmad Gharzi
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Abstract

Background: Group B Streptococcus (GBS) is a bacterium commonly isolated from the vagina. Silver nanoparticles (SNPs) are potential antibacterial agents, and studies have shown their toxic effects. Vitamin C (VC) is an essential vitamin with a protective role against toxicological conditions. We aimed to the evaluation therapeutic effects of the co-administration of SNPs and VC on vaginal infection caused by GBS in mice models. Methods: Vaginitis model was established by intravaginal inoculation of GBS. The Co-administration of SNPs and VC was used to treat the infections. The antibacterial activity of SNPs was determined by the minimum inhibitory concentration. The toxicity of nanoparticles was measured by MTT assay. The microbial load and estrous cycle of mice during treatment were evaluated. Finally, blood samples and vaginal tissue sections were isolated and analyzed. Results: The results showed that SNPs have excellent effects on GBS, and the MIC was 512 ppm. Cell viability after exposure at 512 ppm of SNPs was 32.11% but after treatment with VC increased viability at 512 ppm of nanoparticles to 65.32%. In mice that received SNPs and VC at the same time, the bacteria were completely removed from the vagina, and estrus cycle returned to normal cycle. Analysis of the prepared blood samples and microscopic examination of the vaginal sections confirmed the results. Conclusion: SNPs have a potential antibacterial effect on GBS. But nanoparticles have toxic effects on mammalian cells. The simultaneous use of VC, as a powerful antioxidant, can completely eliminate this toxic effect of nanoparticles.
纳米银与维生素C联合应用治疗B族链球菌阴道感染的疗效观察
背景:B群链球菌(GBS)是一种通常从阴道分离出来的细菌。银纳米颗粒(SNPs)是一种潜在的抗菌药物,研究表明其具有毒性作用。维生素C (VC)是一种必需的维生素,具有预防毒性疾病的作用。我们的目的是评估snp和VC共同给药对小鼠GBS阴道感染的治疗效果。方法:采用阴道内接种GBS建立阴道炎模型。采用snp和VC联合给药治疗感染。以最小抑菌浓度测定SNPs的抑菌活性。采用MTT法测定纳米颗粒的毒性。观察小鼠在治疗期间的微生物负荷和发情周期。最后,分离血液样本和阴道组织切片进行分析。结果:结果表明snp对GBS有良好的影响,MIC为512 ppm。512 ppm snp处理后细胞活力为32.11%,而VC处理后细胞活力为65.32%。在同时接受SNPs和VC的小鼠中,细菌完全从阴道中清除,发情周期恢复正常周期。对准备好的血液样本的分析和阴道切片的显微镜检查证实了这一结果。结论:snp对GBS具有潜在的抗菌作用。但是纳米颗粒对哺乳动物细胞有毒性作用。同时使用VC,作为一种强大的抗氧化剂,可以完全消除纳米颗粒的这种毒性作用。
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来源期刊
Pharmaceutical Sciences
Pharmaceutical Sciences PHARMACOLOGY & PHARMACY-
CiteScore
2.60
自引率
5.90%
发文量
38
审稿时长
4 weeks
期刊介绍: Pharmaceutical Sciences provides a forum for the publication of original research articles, reviews, short communications, and editorials (by invitation only) in all areas of pharmaceutical sciences, including these topics: Clinical Pharmacy Medicinal and Pharmaceutical Chemistry Pharmaceutical Analysis Pharmaceutics Pharmacognosy Pharmacology and Toxicology Pharmaceutical Biotechnology Pharmaceutical Nanotechnology Pharmacoeconomy Radiopharmacy Water, Food, Drug and Cosmetic Control.
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