Effects of Cobalt-60 Gamma on Microbial Elimination and Phytochemical Constituents in Orthosiphon aristatus (Misai Kucing) (Blume) Miq.

IF 0.7 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Sains Malaysiana Pub Date : 2023-07-31 DOI:10.17576/jsm-2023-5207-10

Syafiqah Mhd Jamal, Hasnah Bahari, Daryl Jesus Arapoc, Muhammad Akif Adnan, Imran Jazary Mad Diah, Azmiza Syawani Jasni

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Abstract

Medicinal plants are used for various purposes, however, the presence of microorganisms in them is the main safety risk. The study aimed to evaluate the effects of gamma irradiation on microbial contaminants and phytochemical constituents of Orthosiphon aristatus (Blume) Miq. The plant was irradiated using doses of 3, 6, 9, and 12 kGy and the microbial contamination was assessed using phenotypic and genotypic analyses. The qualitative screening using chemical tests was performed to identify the presence of important phytochemical constituents including alkaloids, saponins, flavonoids, tannins, steroids and triterpenes. Results showed that the total microbial counts in O. aristatus were significantly reduced (P < 0.05) following irradiations at 3- and 6 kGy. Pathogenic bacteria were not detected in O. aristatus after irradiation at 6 kGy while the phytochemical constituents were conserved. In conclusion, gamma irradiation has significantly reduced and eliminated microbial contaminants and preserved the phytochemical constituents of O. aristatus. This study highlights the use of a low and specific dose, 6 kGy that is effective to reduce and eliminate microbial contaminants in O. aristatus.

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钴-60 γ对斜虹吸植物微生物清除和植物化学成分的影响

药用植物用途广泛，但其中微生物的存在是主要的安全隐患。本研究旨在评价γ辐射对正虹吸管(Orthosiphon aristatus (Blume) Miq)微生物污染物和植物化学成分的影响。采用3、6、9和12千吉/小时的剂量照射植株，并利用表型和基因型分析评估微生物污染。使用化学试验进行定性筛选，以确定重要的植物化学成分的存在，包括生物碱、皂苷、黄酮类、单宁、类固醇和三萜。结果表明，马尾莲体内微生物总数显著减少(P <0.05)，在3和6kgy辐照后。6 kGy辐照后，未检出致病菌，植物化学成分保存完好。综上所述，伽马辐照能显著减少和消除马尾莲的微生物污染物，并保存马尾莲的植物化学成分。本研究强调，使用低剂量和特定剂量，6千吉，可以有效地减少和消除麻豆中的微生物污染物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sains Malaysiana MULTIDISCIPLINARY SCIENCES-

CiteScore

1.60

自引率

12.50%

发文量

196

审稿时长

3-6 weeks

期刊介绍： Sains Malaysiana is a refereed journal committed to the advancement of scholarly knowledge and research findings of the several branches of science and technology. It contains articles on Earth Sciences, Health Sciences, Life Sciences, Mathematical Sciences and Physical Sciences. The journal publishes articles, reviews, and research notes whose content and approach are of interest to a wide range of scholars. Sains Malaysiana is published by the UKM Press an its autonomous Editorial Board are drawn from the Faculty of Science and Technology, Universiti Kebangsaan Malaysia. In addition, distinguished scholars from local and foreign universities are appointed to serve as advisory board members and referees.