Polymer-insertion Effect on Urea Conversion Kinetics via Enzymes Immobilized on Magnetic Microparticles

Abstract

Urease was covalently immobilized on the magnetic particles without and with polymer between the urease and the particle. The polymer insertion enhanced the conversion from urea to bicarbonate. The ureases were immobilized on the magnetic microparticles through covalent bonds. 150 μL of stock solution of 3 μm diameter particles was washed three times with 20 mM MES rubbing solution at pH 6.0. The results of this study seem to be useful in designing a reactor for recycling wastewater generated in the semiconductor process.