WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS

IF 1.3 4区农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD

Cellulose Chemistry and Technology Pub Date : 2023-09-29 DOI:10.35812/cellulosechemtechnol.2023.57.72

ALEKSANDAR KNEŽEVIĆ, IVANA ĐOKIĆ, TOMISLAV TOSTI, SLAĐANA POPOVIĆ, DUŠANKA MILOJKOVIĆ-OPSENICA, JELENA VUKOJEVIĆ

{"title":"WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS","authors":"ALEKSANDAR KNEŽEVIĆ, IVANA ĐOKIĆ, TOMISLAV TOSTI, SLAĐANA POPOVIĆ, DUŠANKA MILOJKOVIĆ-OPSENICA, JELENA VUKOJEVIĆ","doi":"10.35812/cellulosechemtechnol.2023.57.72","DOIUrl":null,"url":null,"abstract":"The aim of the study was the comparative analysis of degradation of wheat straw lignin by white-rot fungi and its implications on the efficiency of enzymatic hydrolysis of holocellulose. Peroxidases were found to be predominantly responsible for lignin degradation, even though high laccase activities were detected, especially in the initial stages of fungal culturing. The studied fungal species showed various ability to degrade lignin in wheat straw, which further affected the release of reducing sugars during enzymatic saccharification. The highest rate of lignin, hemicelluloses and cellulose degradation was noticed in the sample pretreated with Irpex lacteus. Among all the tested species, only Ganoderma resinaceum was found as a suitable lignin degrader, with 2-fold higher hydrolysis yield (51.1 ± 4.7%) than in the control. A key mechanism that enhances convertibility of carbohydrates is the selective lignin removal from biomass. Operating time, holocellulose loss and unpredictable fungal response to culturing conditions are the main challenges in fungal pretreatment of lignocellulosic feedstock.","PeriodicalId":10130,"journal":{"name":"Cellulose Chemistry and Technology","volume":"31 1","pages":"0"},"PeriodicalIF":1.3000,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cellulose Chemistry and Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35812/cellulosechemtechnol.2023.57.72","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, PAPER & WOOD","Score":null,"Total":0}

引用次数: 0

Abstract

The aim of the study was the comparative analysis of degradation of wheat straw lignin by white-rot fungi and its implications on the efficiency of enzymatic hydrolysis of holocellulose. Peroxidases were found to be predominantly responsible for lignin degradation, even though high laccase activities were detected, especially in the initial stages of fungal culturing. The studied fungal species showed various ability to degrade lignin in wheat straw, which further affected the release of reducing sugars during enzymatic saccharification. The highest rate of lignin, hemicelluloses and cellulose degradation was noticed in the sample pretreated with Irpex lacteus. Among all the tested species, only Ganoderma resinaceum was found as a suitable lignin degrader, with 2-fold higher hydrolysis yield (51.1 ± 4.7%) than in the control. A key mechanism that enhances convertibility of carbohydrates is the selective lignin removal from biomass. Operating time, holocellulose loss and unpredictable fungal response to culturing conditions are the main challenges in fungal pretreatment of lignocellulosic feedstock.

查看原文本刊更多论文

小麦秸秆白腐真菌预处理:对碳水化合物聚合物酶解的影响

本研究的目的是比较分析白腐菌对麦草木质素的降解及其对全纤维素酶解效率的影响。尽管在真菌培养的初始阶段检测到高漆酶活性，但也发现过氧化物酶主要负责木质素的降解，特别是在真菌培养的初始阶段。所研究的真菌种类表现出不同的降解麦草木质素的能力，这进一步影响了酶解糖化过程中还原糖的释放。木质素、半纤维素和纤维素的降解率在用Irpex lateus预处理的样品中最高。在所有被试树种中，只有树脂灵芝(Ganoderma resinaceum)是合适的木质素降解剂，其水解率(51.1±4.7%)是对照的2倍。提高碳水化合物可转换性的一个关键机制是选择性地去除生物质中的木质素。操作时间、整体纤维素损失和不可预测的真菌对培养条件的反应是木质纤维素原料真菌预处理的主要挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cellulose Chemistry and Technology 工程技术-材料科学：纸与木材

CiteScore

2.30

自引率

23.10%

发文量

审稿时长

7.3 months

期刊介绍： Cellulose Chemistry and Technology covers the study and exploitation of the industrial applications of carbohydrate polymers in areas such as food, textiles, paper, wood, adhesives, pharmaceuticals, oil field applications and industrial chemistry. Topics include: • studies of structure and properties • biological and industrial development • analytical methods • chemical and microbiological modifications • interactions with other materials