Rabiha Q. Thajel, Abdulameer M. Ghareeb, Qays A. M. Al-Khafaji
{"title":"Assessing miR-146a-5P with IL-6 and TLR-4 serum levels in diabetic mellitus patients with or without urinary tract infection","authors":"Rabiha Q. Thajel, Abdulameer M. Ghareeb, Qays A. M. Al-Khafaji","doi":"10.51248/.v43i5.3258","DOIUrl":null,"url":null,"abstract":"Introduction and Aim: Diabetes is a chronic metabolic disease that affects glucose levels. This condition increases blood sugar and weakens immunity, damaging neutrophil function and urine antibacterial activity. IL-6 and TLR-4 are involved in inflammation and immune system control, while miR-146-5p may be a biomarker for diabetes. This study examines IL-6, TLR-4, and miR-146-5p in diabetics with and without UT infection. Materials and Methods: In this study, blood samples were collected from 100 diabetic mellitus patients suffering from UTI in addition to 100 individuals apparently healthy as control. The serum levels of IL-6 and TLR-4 were detected using commercial BTlab brand ELISA kits. The plasma RNA expression level of the miR-146a-5p was determined by real time-PCR using a specific primer for miR-146a-5p with the U6 gene utilized as a reference gene. Results: The results showed that diabetic patient’s group had a significantly higher response of IL-6 than controls (75.6 vs. 27.6). TLR-4 levels showed high levels in diabetic patients’ group (1.3) in comparison with the control group (0.441). Both IL-6 and TLR-4 levels showed a significant difference between the diabetic patients with UTI compared to control. The results revealed no-significant differences in the levels among both diabetic patients with UTI and patients without UTI. TLR-4 and IL-6 showed a significant inverse relationship while the relation between TLR-4 level and miR-146a-5p expression level showed a negative relationship. Conclusion: The levels of IL-6, TLR-4, and miR-146-5p expression were found to be considerably greater in individuals with diabetes having UTIs compared to patients with diabetes alone. An inverse relationship existed between IL-6 and TLR-4 levels as well as between TLR-4 and miR-146a-5p. The expression levels of IL-6 and miR-146-5p were nearly equal.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicine (India)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.51248/.v43i5.3258","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction and Aim: Diabetes is a chronic metabolic disease that affects glucose levels. This condition increases blood sugar and weakens immunity, damaging neutrophil function and urine antibacterial activity. IL-6 and TLR-4 are involved in inflammation and immune system control, while miR-146-5p may be a biomarker for diabetes. This study examines IL-6, TLR-4, and miR-146-5p in diabetics with and without UT infection. Materials and Methods: In this study, blood samples were collected from 100 diabetic mellitus patients suffering from UTI in addition to 100 individuals apparently healthy as control. The serum levels of IL-6 and TLR-4 were detected using commercial BTlab brand ELISA kits. The plasma RNA expression level of the miR-146a-5p was determined by real time-PCR using a specific primer for miR-146a-5p with the U6 gene utilized as a reference gene. Results: The results showed that diabetic patient’s group had a significantly higher response of IL-6 than controls (75.6 vs. 27.6). TLR-4 levels showed high levels in diabetic patients’ group (1.3) in comparison with the control group (0.441). Both IL-6 and TLR-4 levels showed a significant difference between the diabetic patients with UTI compared to control. The results revealed no-significant differences in the levels among both diabetic patients with UTI and patients without UTI. TLR-4 and IL-6 showed a significant inverse relationship while the relation between TLR-4 level and miR-146a-5p expression level showed a negative relationship. Conclusion: The levels of IL-6, TLR-4, and miR-146-5p expression were found to be considerably greater in individuals with diabetes having UTIs compared to patients with diabetes alone. An inverse relationship existed between IL-6 and TLR-4 levels as well as between TLR-4 and miR-146a-5p. The expression levels of IL-6 and miR-146-5p were nearly equal.
简介和目的:糖尿病是一种影响血糖水平的慢性代谢疾病。这种情况会使血糖升高,免疫力减弱,损害中性粒细胞功能和尿液抗菌活性。IL-6和TLR-4参与炎症和免疫系统控制,而miR-146-5p可能是糖尿病的生物标志物。本研究检测了伴有和不伴有UT感染的糖尿病患者的IL-6、TLR-4和miR-146-5p。材料与方法:本研究采集了100例糖尿病尿路感染患者的血液样本,同时采集了100例表面健康的患者作为对照。采用商用BTlab品牌ELISA试剂盒检测血清IL-6和TLR-4水平。采用miR-146a-5p特异性引物,以U6基因作为内参基因,real - time-PCR检测miR-146a-5p的血浆RNA表达水平。结果:糖尿病患者组IL-6应答明显高于对照组(75.6 vs. 27.6)。与对照组(0.441)相比,糖尿病组TLR-4水平较高(1.3)。糖尿病合并UTI患者与对照组相比,IL-6和TLR-4水平均有显著差异。结果显示合并UTI的糖尿病患者和未合并UTI的糖尿病患者的水平无显著差异。TLR-4与IL-6呈显著负相关,而TLR-4水平与miR-146a-5p表达水平呈负相关。结论:与糖尿病患者相比,合并尿路感染的糖尿病患者IL-6、TLR-4和miR-146-5p的表达水平明显更高。IL-6与TLR-4、TLR-4与miR-146a-5p呈负相关。IL-6和miR-146-5p的表达水平几乎相等。