Exploring genetic polymorphisms and transcript levels of antioxidant and metabolic markers for prediction and monitoring diarrhea in Holstein dairy calves

Pub Date : 2023-11-01 DOI:10.1080/09064702.2023.2274881

Ahmed Ateya, Mona Al-Sharif, Salah H. Faraj, Mohamed Abdo, Liana Fericean, Ioan Banatean-Dunea, Salam J. Hammad, Maha Mamdouh, Hayat Fayed, Marawan A. Marawan, Walaa S. Raslan

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Abstract

The objective of this study was to investigate the genetic polymorphisms and transcript levels of antioxidant and metabolic markers for diarrhea monitoring in Holstein dairy calves. For DNA and RNA extraction, blood samples were taken from 100 female calves (50 with diarrhea and 50 appearing healthy). DNA sequencing and real time PCR for and metabolic genes were performed to identify genetic variants linked to diarrhea susceptibility. Results revealed that there were nucleotide sequence variations in the investigated genes between healthy and diarrheal calves. Keap1, HMOX1, CMPK2, ASPG, FPGT, TNNI3 K, and LPCAT1 were considerably more highly expressed in diarrheic calves than resistant ones. The Nrf2, PRDX2, and PRDX6 genes, however, produced a different pattern. This study provides a practical control strategy to lessen newborn diarrhea in Holstein dairy calves and emphasizes the significance of nucleotide variations along with antioxidant and metabolic gene expression patterns as proxy markers of diarrhea susceptibility/resistance.

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探索用于预测和监测荷斯坦奶牛犊牛腹泻的抗氧化和代谢标志物的遗传多态性和转录水平

本研究的目的是研究荷斯坦奶牛犊牛腹泻监测中抗氧化和代谢标志物的遗传多态性和转录水平。为了提取DNA和RNA，采集了100头母牛犊的血液样本(50头患有腹泻，50头看起来健康)。对代谢基因进行DNA测序和实时PCR，以确定与腹泻易感性相关的遗传变异。结果显示，健康犊牛与腹泻犊牛之间存在核苷酸序列差异。Keap1、HMOX1、CMPK2、ASPG、FPGT、tnni3k和LPCAT1在腹泻犊牛中的表达明显高于耐药犊牛。然而，Nrf2、PRDX2和PRDX6基因产生了不同的模式。本研究为减少荷斯坦奶牛犊牛新生儿腹泻提供了一种实用的控制策略，并强调了核苷酸变异以及抗氧化和代谢基因表达模式作为腹泻易感性/耐药性的替代标记的重要性。

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