Homeologue differential expression in the flavonoid biosynthetic pathway underlies flower colour variation in natural and synthetic polyploids of Nicotiana tabacum (Solanaceae)

IF 2.3 2区 生物学 Q2 PLANT SCIENCES
Elizabeth W McCarthy, Jacob B Landis, Abigail G McCoy, Amber J Lawhorn, Amelda Kurti, Yimin Xu, James J Giovannoni, Amy Litt
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引用次数: 1

Abstract

Abstract Homeologue expression bias occurs when one progenitor copy of a gene is expressed at a higher level than the other in allopolyploids. Morphological variation, including differences in flower colour, exists between natural and synthetic allopolyploids of Nicotiana tabacum and their progenitors. In this study, we use a comparative transcriptomic approach to investigate gene expression differences as well as homeologue bias in the flavonoid biosynthetic pathway (FBP) in these accessions. We do not observe reciprocal homeologue bias between dark and light pink allopolyploids, but the production of light pink flowers is correlated with high FLAVONOL SYNTHASE:DIHYDROFLAVONOL-4-REDUCTASE (FLS:DFR) ratio at 60% of anthesis length due to delayed activation of DFR in these accessions. We do find that natural allopolyploids have stronger homeologue bias than synthetic allopolyploids in both FBP genes and across the transcriptome. While there is no overall subgenome dominance, there is a bias towards expression of N. tomentosiformis homeologues in FBP genes; however, the magnitude of this bias is reduced in allopolyploids compared to the progenitors, suggesting that N. sylvestris homeologues play an active role in the development of flower colour in N. tabacum allopolyploids. In addition, synthetic allopolyploids tend to exhibit trans regulation of homeologues whereas natural allopolyploids often have evolved cis-regulatory differences between homeologues since their origin.
黄酮类化合物生物合成途径的同源性差异表达是烟草天然多倍体和人工多倍体花色变化的基础
当一个基因的一个祖先拷贝在异源多倍体中表达的水平高于另一个基因时,就会发生同源表达偏倚。烟草的天然异源多倍体和人工异源多倍体及其祖体细胞之间存在形态变异,包括花色的差异。在这项研究中,我们使用比较转录组学方法来研究这些植物中黄酮类生物合成途径(FBP)的基因表达差异和同源性偏差。我们没有观察到深粉红色和浅粉红色异体多倍体之间的反向同源性偏向,但由于DFR的延迟激活,这些材料在花长60%时产生浅粉红色的花与高黄酮醇合成酶:二氢黄酮醇-4-还原酶(FLS:DFR)比例相关。我们确实发现,在FBP基因和整个转录组中,天然异源多倍体比合成异源多倍体具有更强的同源性偏倚。虽然没有整体的亚基因组优势,但在FBP基因中有一种倾向于表达绒毛状奈米虫同源物的倾向;然而,与祖先相比,异源多倍体的这种偏倚程度有所降低,这表明sylvestris同源物在烟草异源多倍体的花色发育中起着积极的作用。此外,合成的异源多倍体往往表现出同源物的反式调控,而天然的异源多倍体自起源以来往往在同源物之间进化出顺式调控差异。
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来源期刊
CiteScore
5.70
自引率
8.30%
发文量
54
审稿时长
3.5 months
期刊介绍: The Botanical Journal of the Linnean Society publishes original papers on systematic and evolutionary botany and comparative studies of both living and fossil plants. Review papers are also welcomed which integrate fields such as cytology, morphogenesis, palynology and phytochemistry into a taxonomic framework. The Journal will only publish new taxa in exceptional circumstances or as part of larger monographic or phylogenetic revisions.
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