Functionalized SWCNTs@Ag–TiO2 nanocomposites induce ROS-mediated apoptosis and autophagy in liver cancer cells

IF 6.1 3区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Majid S. Jabir, Mustafa K. A. Mohammed, Salim Albukhaty, Duha S. Ahmed, Asad Syed, Abdallah M. Elgorban, Rajalakshmanan Eswaramoorthy, Hayder M. Al-kuraishy, Ali I. Al-Gareeb, Suresh Ghotekar, Sabrean F. Jawad, Mazin A. A. Najm
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Abstract

Abstract Hybrid nanomaterials with unique physiochemical properties have received a lot of attention, making them attractive for application in different fields like cancer treatment. This study was designed to investigate the combined effects of single-walled carbon nanotubes (SWCNTs) hybridized with silver titanium dioxide composite (SWCNTs@Ag–TiO 2 ). Transmission electron microscopy and field emission scanning electron microscopy images demonstrated the accumulation of SWCNTs with Ag–TiO 2 due to an increased main grain size with functionalization to 40 nm. The D and G bands in SWCNTs @Ag–TiO 2 shifted to 1,366 and 1,534 cm −1 , respectively. SWCNTs@Ag-TiO 2 were assessed for their cytotoxicity and autophagy induction in liver cancer cells (Hep-G2) using the lactate dehydrogenase assay, MTT assay, and flow cytometry methods. The results showed that SWCNTs and SWCNTs@Ag–TiO 2 exhibited strong anti-cancer activity in vitro against Hep-G2 cells by inducing apoptosis and autophagy in liver cancer cells via controlling the AKT and JNK mitogen-activated protein kinase pathways. The results show that SWCNTs and SWCNTs coated with silver/titanium dioxide (SWCNTs@Ag–TiO 2 ) reduce the cells’ viability and proliferation. It was shown that an excessive amount of reactive oxygen species was a crucial mediator of both the cell death caused by SWCNTs and the cell death caused by SWCNTs combined with Ag–TiO 2 . Based on these findings, it appears that SWCNTs and SWCNTs@Ag–TiO 2 have the potential to be developed as nanotherapeutics for the treatment of liver cancer cells.
功能化SWCNTs@Ag -TiO2纳米复合材料诱导ros介导的肝癌细胞凋亡和自噬
摘要杂化纳米材料以其独特的物理化学性质受到广泛关注,在癌症治疗等不同领域具有广泛的应用前景。本研究旨在研究单壁碳纳米管(SWCNTs)与银二氧化钛复合材料(SWCNTs@Ag - tio2)杂交的综合效应。透射电子显微镜和场发射扫描电子显微镜图像显示,由于主晶粒尺寸随着功能化增加到40 nm, ag - tio2在SWCNTs中积累。SWCNTs @ ag - tio2中的D带和G带分别位移到1366和1534 cm−1。使用乳酸脱氢酶测定、MTT测定和流式细胞术方法评估SWCNTs@Ag-TiO 2对肝癌细胞(Hep-G2)的细胞毒性和自噬诱导。结果表明,SWCNTs和SWCNTs@Ag - tio2通过控制AKT和JNK丝裂原激活的蛋白激酶途径诱导肝癌细胞凋亡和自噬,在体外对Hep-G2细胞表现出较强的抗癌活性。结果表明,SWCNTs和包裹银/二氧化钛(SWCNTs@Ag - tio2)的SWCNTs降低了细胞的活力和增殖能力。研究表明,过量的活性氧是SWCNTs引起的细胞死亡和SWCNTs与ag - tio2联合引起的细胞死亡的关键介质。基于这些发现,SWCNTs和SWCNTs@Ag - tio2似乎具有开发用于肝癌细胞治疗的纳米疗法的潜力。
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来源期刊
Nanotechnology Reviews
Nanotechnology Reviews CHEMISTRY, MULTIDISCIPLINARY-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
11.40
自引率
13.50%
发文量
137
审稿时长
7 weeks
期刊介绍: The bimonthly journal Nanotechnology Reviews provides a platform for scientists and engineers of all involved disciplines to exchange important recent research on fundamental as well as applied aspects. While expert reviews provide a state of the art assessment on a specific topic, research highlight contributions present most recent and novel findings. In addition to technical contributions, Nanotechnology Reviews publishes articles on implications of nanotechnology for society, environment, education, intellectual property, industry, and politics.
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