Sheep spermatogenesis is affected by the nutritional levels of cocoa meal, a byproduct of cocoa butter manufacturing rich in theobromine and caffeine

Diego Silva Macedo, Laiara Fernandes Rocha, Emmanuel Emydio Gomes Pinheiro, Ana Lúcia Almeida Santana, Rosileia Silva Souza, Adriana Regina Bagaldo, Poliana Plácido Almeida Bezerra, Larissa Pires Barbosa
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Abstract

The objective of this study was to evaluate the effects of cocoa meal in feed concentrate on the spermatogenesis in sheep. Twenty-five uncastrated males were divided into four groups and fed concentrated feed, supplemented with 0, 10, 20, and 30% cocoa meal, respectively, for 150 days. At the end of this period, the animals were slaughtered, the testicles were collected for histological slides, and testicular morphometric assessments were conducted, including cell type quantification in the seminiferous epithelium, intrinsic spermatogenesis [...]
绵羊的精子发生受可可粉营养水平的影响,可可粉是富含可可碱和咖啡因的可可脂生产的副产品
本研究的目的是评价浓缩饲料中可可粉对绵羊精子发生的影响。将25只未阉割的雄鼠分为4组,分别饲喂添加0、10、20和30%可可粉的浓缩饲料,饲喂150 d。在这段时间结束时,屠宰动物,收集睾丸进行组织学切片,并进行睾丸形态计量学评估,包括精子上皮细胞类型定量,内在精子发生[…]
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