Molecular Identification and Characterization of a Begomovirus Associated with Okra Enation Leaf Curl Disease in Mali

Gaoussou K. Keita, Laya Kansaye, Lassina Doumbia, Ibrahim Keita, Mariam Sangare, Tidiani Sinayoko, Boubacar Macalou, Moussa Noussourou Maiga, Nadou Paul Sanogo, Ousmane Koita
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Abstract

Okra is one of the most widespread vegetable crops in the world, particularly in West Africa. However, several factors influence okra crops as biotic and abiotic factors. Among the diseases affecting its culture, okra leaf curl disease is a major threat. This study aims to assess begomoviruses from okra plants with symptoms like leaves curl by molecular approach. A total of thirteen serologically positive samples were tested by PCR assay, and one sample was sequenced among them. The begomovirus was found in six isolates, Bhendi yellow vein mosaic virus (BYVMV) in three isolates, and Okra enation leaf curl virus (OELCuV) in three isolates, respectively. The begomovirus isolate sequences shared 90% identity with the Cotton leaf curl Gezira virus. Mixed infections between these viruses were found. Thus, these results highlight the need to monitor the spread of these disease-causing viruses for okra crops in Mali. In addition, they can also lead to a considerable loss in okra fields in Koulikoro, which is an agricultural region by excellence.
一种与马里秋葵成虫卷曲病相关的begomavirus的分子鉴定和特性分析
秋葵是世界上分布最广的蔬菜作物之一,尤其是在西非。然而,影响秋葵作物生长的因素有生物因素和非生物因素。在影响秋葵栽培的病害中,秋葵卷叶病是主要威胁。本研究旨在通过分子方法评估具有叶片卷曲等症状的秋葵属植物的begomovirus。对13份血清学阳性标本进行PCR检测,并对其中1份标本进行测序。6株分离物中检出begomvirus, 3株分离物中检出Bhendi yellow vein mosaic virus (BYVMV), 3株分离物中检出Okra enation leaf curl virus (OELCuV)。begomvirus分离物序列与棉花卷叶病毒有90%的同源性。发现了这些病毒之间的混合感染。因此,这些结果强调了监测这些致病病毒在马里秋葵作物传播的必要性。此外,它们还可能导致库利科罗的秋葵田遭受相当大的损失,而库利科罗是一个卓越的农业地区。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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