The Effect of nutritional and physical factors on the growth of Trametes versicolor (L.) Lloyd and its mycochemical and cytotoxic properties

Q4 Environmental Science

Studies in Fungi Pub Date : 2023-01-01 DOI:10.48130/sif-2023-0018

Johnmel A. Fabros, Mark Kevin M. Lazo, Janah Elvira S. Magpantay, Marcelino D. Abon, Rich Milton R. Dulay, Sofronio P. Kalaw, Renato G. Reyes

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Abstract

Trametes versicolor (L.) Lloyd is an agaricomycetous fungi characterized by its fan-shaped or shelf-like fruiting bodies with a thin leathery and velvety upper surface that displays concentric bands of brown-black and white color and undersurface cap features with numerous tiny spores. In this study, the culture condition for the mycelial growth as well as the fruiting body production was established. The mycochemical compositions and the cytotoxic activity were also elucidated. Optimization study of the secondary mycelia shows that T. versicolor luxuriantly grew on malt extract agar (MEA), potato dextrose agar (PDA), and coconut water gulaman (CWG) culture media. In terms of pH of the medium, pH 6.0 to 8.0 supports the best mycelial growth. On the other hand, sealed, lighted conditions incubated at 25 °C to 32 °C were the requirements for the optimum growth of T. versicolor. After 37 days of incubation period, the fruiting body production of T. versicolor was determined. T. versicolor produced 37.68 g bag⁻¹ which is equivalent to 7.65% biological efficiency (BE) on a substrate consisting of rice straw and sawdust at 7:3 ratio by volume. Moreover, qualitative mycochemical analysis of the aqueous extract of T. versicolor revealed the presence of different mycochemicals such as terpenoids, flavonoids, tannins, saponins, and alkaloids. In terms of the cytotoxic effect of T. versicolor, the LC50 values of ethanol and methanol extracts were calculated, showing that they had a high toxicity level of 70.93 µg/ml and 74.43 µg/ml, respectively against brine shrimp nauplii after 24 hours of incubation. Overall, the optimum culture condition for mycelial growth and fruiting body production, mycochemical compounds, and cytotoxic effect of T. versicolor tabulated in this study provide significant data that elucidated the value of this mushroom in the pharmaceutical industries.

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营养和物理因素对彩板草生长的影响(l)劳埃德及其真菌化学和细胞毒性

彩板草(L.)劳埃德是一种琼脂菌属真菌，其特点是其扇形或架子状的子实体，其薄革质和天鹅绒状的上表面显示棕黑色和白色的同心带，其下表面有帽状特征，有许多微小的孢子。在本研究中，建立了菌丝生长和子实体产生的培养条件。并对其真菌化学成分和细胞毒活性进行了分析。对次生菌丝体的优化研究表明，在麦芽膏琼脂(MEA)、马铃薯葡萄糖琼脂(PDA)和椰子水古拉曼(CWG)培养基上，花色T. versicolor生长繁茂。培养基的pH值为6.0 ~ 8.0时菌丝生长最佳。在25 ~ 32℃的密闭光照条件下，花椒生长最佳。经过37 d的孵育，测定了花色霉的子实体产量。在稻秆与木屑体积比为7:3的基质上，花色t产生37.68 g bag−1，相当于7.65%的生物效率(BE)。此外，对花椒水提物进行真菌化学定性分析，发现花椒水提物中含有萜类、黄酮类、单宁类、皂苷类和生物碱等真菌化学物质。在细胞毒作用方面，计算了不同乙醇提取物和甲醇提取物的LC50值，结果表明，在孵育24小时后，其对盐水虾nauplii的毒性分别为70.93µg/ml和74.43µg/ml。总的来说，本研究中列出的菌丝生长和子实体产生的最佳培养条件、真菌化学成分和细胞毒性作用提供了重要的数据，阐明了这种蘑菇在制药工业中的价值。

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