Metabolic Stability of Eight Airborne OrganoPhosphate Flame Retardants (OPFRs) in Human Liver, Skin Microsomes and Human Hepatocytes

IF 2.5 4区工程技术 Q3 CHEMISTRY, ANALYTICAL

Separations Pub Date : 2023-10-25 DOI:10.3390/separations10110548

Stefano Di Bona, Emanuele Artino, Francesca Buiarelli, Patrizia Di Filippo, Roberta Galarini, Stefano Lorenzetti, Franco Lucarelli, Gabriele Cruciani, Laura Goracci

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引用次数: 0

Abstract

The waste of electrical and electronic equipment (WEEE) is generally considered a secondary raw material for the recovery of valuable components. However, emerging issues regarding the impact of suspended particles arising from WEEE recycling operations are a concern. It was recently demonstrated that samples from three different WEEE plants were rich in organophosphate flame retardants (OPFRs). Since exposure to a xenobiotic can lead to its biotransformation through human metabolism routes, in the present study, the metabolism of eight OPFRs of interest in our sampling campaign (triphenyl phosphate (TPhP), tri-m-tolyl phosphate (TMTP), ethylhexyl diphenyl phosphate (EHDPhP), tributoxyethyl phosphate (TBOEP), diphenyl phosphate (DPhP), trichloroethyl phosphate (TCEP), tris(1,3-dichloropropan-2-yl) phosphate (TDClPP) and bisphenol A bis(diphenyl phosphate) (BDP)) was investigated. Their metabolism was studied at different time points in three matrices: human liver microsomes, human hepatocytes and human skin microsomes. This study, which was run using a common experimental setting, allowed easy comparison of results for each OPFR of interest, and a comparison with other data in the literature was performed. In particular, a number of metabolites not previously described were detected, and for the first time, it was shown that TPhP could be metabolized in human skin microsomes.

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八种空气传播有机磷阻燃剂(OPFRs)在人体肝脏、皮肤微粒体和肝细胞中的代谢稳定性

电气和电子设备(WEEE)的废物通常被认为是回收有价值部件的二次原材料。然而，关于报废电子电气设备回收作业产生的悬浮颗粒影响的新问题是一个值得关注的问题。最近有研究表明，来自三种不同WEEE工厂的样品富含有机磷阻燃剂(OPFRs)。由于暴露于外源生物可导致其通过人体代谢途径进行生物转化，因此在本研究中，研究了我们采样活动中感兴趣的八种OPFRs(三苯基磷酸(TPhP)，三-间甲基磷酸(TMTP)，乙基己基磷酸(EHDPhP)，三氧乙基磷酸(TBOEP)，二苯基磷酸(DPhP)，三氯乙基磷酸(TCEP)，三(1,3-二氯丙基-2-基)磷酸(TDClPP)和双酚a -二(二苯基磷酸)(BDP))的代谢。研究了它们在人肝微粒体、人肝细胞和人皮肤微粒体三种基质中不同时间点的代谢。本研究采用通用实验设置，便于对每个感兴趣的OPFR的结果进行比较，并与文献中的其他数据进行比较。特别是，许多以前未描述的代谢物被检测到，并且首次表明TPhP可以在人体皮肤微粒体中代谢。

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来源期刊

Separations Chemistry-Analytical Chemistry

CiteScore

3.00

自引率

15.40%

发文量

342

审稿时长

12 weeks

期刊介绍： Separations (formerly Chromatography, ISSN 2227-9075, CODEN: CHROBV) provides an advanced forum for separation and purification science and technology in all areas of chemical, biological and physical science. It publishes reviews, regular research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: Manuscripts regarding research proposals and research ideas will be particularly welcomed. Electronic files and software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. Manuscripts concerning summaries and surveys on research cooperation and projects (that are funded by national governments) to give information for a broad field of users. The scope of the journal includes but is not limited to: Theory and methodology (theory of separation methods, sample preparation, instrumental and column developments, new separation methodologies, etc.) Equipment and techniques, novel hyphenated analytical solutions (significantly extended by their combination with spectroscopic methods and in particular, mass spectrometry) Novel analysis approaches and applications to solve analytical challenges which utilize chromatographic separations as a key step in the overall solution Computational modelling of separations for the purpose of fundamental understanding and/or chromatographic optimization